白花丹素聯(lián)合多西他賽對前列腺癌細胞的增殖抑制及其機制研究.pdf

1、華中科技大學(xué)碩士學(xué)位論文白花丹素聯(lián)合多西他賽對前列腺癌細胞的增殖抑制及其機制研究姓名：劉慶申請學(xué)位級別：碩士專業(yè)：外科學(xué)（泌尿外科）指導(dǎo)教師：鞠文201104華中科技大學(xué)碩士學(xué)位論文華中科技大學(xué)碩士學(xué)位論文2關(guān)鍵詞：白花丹素；多西他賽；前列腺癌；凋亡；NFκB，Bcl2BaxVegfAbstractObjective:Investigatedtheeffectpotentialmechanismofcombinedplumbagindo

2、cetaxelonapoptosisgrowthinhibitionofprostatecancercellinvitro.Methods:ProstatecancercellsPC3wereusedwhichwereromizedintofourgroups.Oneusedascontrolsgroup.(Cgroup)theothersweretreatedwithplumbagin(Pgroup5M)docetaxel(Dgrou

3、p5nM)theircombinationindividually(PD).Aftercultivationf24~48hrsthegrowthinhibitionratesofPC3cellswerestudiedbyMTTcolimetry.CellularapoptosiswasdetectedbyacridineangeethidiumbroefluescentstainingAnnexinVFITCpropidiumiodid

4、estainingflowcytometry(FCM).ThemRNAproteinlevelsofNFκBBcl2BaxVegfweredeterminedbyRTPCRwesternblotting.Results:EachinterventiongroupcouldinhibitthegrowthofPC3inadosetimedependentmanner.TheproliferationratesofDgroupPgroupD

5、Pgroupin24hwere19.11%，25.23%46.53%respectively.TheinhibitingrateinDPgroupwassignificantlyhigherthanothergroups(P0.01P0.05).TheapotosisratesofDgroupPgroupDPgroupin24hwere5.1%20.5%29.4%51.2%.TheapotosisrateinDPgroupwassign

6、ificantlyhigherthanothers(P0.05).Partialcellspresentedtheacteristicmphologicalchangesofapotosis.ThemRNAproteinlevelsofNFκBBcl2VegfinDPgroupweresignificantlyreducedthanothershoweverthemRNAproteinlevelofBaxwasupregulated.C

7、onclusion:PlumbaginplusdocetaxelcouldsignificantlyinhibittheviabilityofPC3cellsbysuppressingproliferationinduingapotosisitsmechanismsmightbeinvovlvedinthedownregulationofNFκBBcl2VegfupregulationofBaxgene.KeyWds:Plumbagin