不同濃度富血小板血漿應(yīng)用于骨組織工程的研究.pdf

1、青島大學(xué)碩士學(xué)位論文不同濃度富血小板血漿應(yīng)用于骨組織工程的研究姓名：潘永海申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：口腔臨床醫(yī)學(xué)（頜面外科）指導(dǎo)教師：李寧毅20060513APPLICATIONoFPLATELETRICHPLASMAoFDIFFERENTCONCENTRATloNSINBoNETISSUEENGINEERING【Abstract1ObjectiveToobserveinvitrotheeffectsofplateletriehplasm

2、a(PRP)ofdifferentconcentrationsontheproliferation，differentiation，andmRNAofautologousboneinarrowstromalcells(MSCs)toevaluatetheboneinductioneffectofH啦onMSCs，andtoinvestigatethefeasibilityofbonetissueregenerationbytissuee

3、ngineeringwi也theobtainedoptimalconcentrationMethodsTwotimesofcentrifugationwasusedtoextractPRPfromthevenousbloodofthedogsandthebloodcountwasperformedfnleserumrichingrowthfactors(SRGF)wereactivatedandpreparedwithserumfree

4、D—MEM／F12solutiontogetl％，5％，10％，20％，and30％SRGFDensitygradientcenlrifugationWasappliedtOextractandcultureMSCsofthedogsandgeneratedwhichWasobservedandphotographedeverydayTheeffectsofPRPofdifferentconeentrations(1％，5％，10％，2

5、0％，and30％)onMSCsproliferationwe陀observedwithMITondays1，3，5，7，and9EnzymekineticswasusedtOd鼬ecttheeffectofPRPofdifferentconcentrationsOnALPactivityondays3，6，9，and12ALPstainingwasperformedwithimprovedOomoristainingtestonPRP

6、indncedMSCs耵七formationof加in啪Ⅱ蹬dnoduleswasdetectedwithDam]vfcGl鷺Russll$alizarinredstainThecontentsofosteoealcinwerc0bservedondays7，1421，and28Semiquantitative“I、圯讎廿anseripfionpolymerasechainreaction(RTPCm)wasusedtodetectth

7、eAI，PmRNAandoCNmRNAlevelsinPRPandrhBMPinducedMSCsResultsAdherenceoftheMS(=saftertheprimaryculturebegantoOeCIIl“at2448handthecellsblendedintoamonolayer12dlaterMostofthecellsassumedfibroblastfusiformcellsAftertheinductionw

8、ithPRPthecellsincreasedsignificantlyandgraduallychangedintosquareOrpolygonandmeltedintomonolayer鉗dlaterandmultilayer812dlatern特plateletcountshowedthattheplateletconcentrationinPRPWas538timesthatinthewholeblood^d1Tdetecti

9、onshowedthatvariouslevelsofproliferationoccurredinallthePRPgroupsespeciallyinthe10％PRPgroupEnzymekineticsdetectionshowedthattheAIJPactivityincreasedinallthePRPgroupsespeciallyinthe10％PRPandrhBMPgroupsForthePRPinducedMSCs