高效親和色譜法對(duì)四種中藥活性成分與人血清白蛋白相互作用的研究.pdf

1、西北大學(xué)碩士學(xué)位論文高效親和色譜法對(duì)四種中藥活性成分與人血清白蛋白相互作用的研究姓名：于雪申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：衛(wèi)引茂張君才20100613西北大學(xué)碩士學(xué)位論文StudyontheInteractionbetweenFourActiveComponentsofChineseHerbandHumanSerumAlbumbyHighperformanceAffinityChromatographyAbstractThei

2、nteractionofdrugsandproteininbloodCaninfluencedrugtransportation，distribution，metabolism，andexcretionInaddition，thedirectorindirectcompetitionbetweentwoormoredrugsforthesamebindingproteinshasbeenknowasasourceofdrug—drugi

3、nteractionwhichcallpossiblyaffectthedispositionofseveraldrugs，andresultinmanyphysiologicalconsequencesTherefore，thestudyofdrugproteinisofgreatsignificanceHumanserumalbumin(HSA)andal—acidglycoprotein(AGP)arethemostimporta

4、ntplasmaproteinstoactasdrugcarder，andoftenusedtostudythebindinginformationfordrugInthistext，highperformanceaffinitychromatographyWasusedtoinvestigatethebindingoffourdrugstoimmobilizedHSAcolumnbyfrontalanalysisandzonalelu

5、tionexperimentsundertheconditionofpHof74andtemperatureof37℃，thatisregardedaSphysicalconditionTheobtainedconclusionare1HSAwasimmobilizedontotheaminopropylsilicaunderamildconditionTheHSAcolumnWaSprovedtobondonthesurfaceofs

6、ilicawithchiralcompoundasatest2Frontalanalysiswasperformedtodeterminetheadsorptivecapacityoffourdrugspaeonol，ferulicacid，chlorogenicacidandvanillicacidonHSAcolumnTheexperimentdatawereanalysedbyusingone—sitemodel，twositem

7、odelanddouble—reciprocalfrontalanalysisplotrespectivelyTheresultsshowedthatonlyonemajorbindingsiteonHSAmolecularWasprovidedfortheinteractionofpaeonol，ferulicacidandvanillicacid晰tllHSA，butchlorogenicacidgaveabetterfittoth

8、etwo—sitemodel，itindicatedthatchlorogenicacidhadmorethanonebindingsiteonHSA3Competitionstudiesbasedonzonalelutionbetweenpaeonol，ferulicacid，chlorogenicacid，vanillicacidandfourprobecompoundswerecarriedouttoinvestigatebind