小鼠子宮巨噬細(xì)胞的分離、鑒定及其在妊娠早期的變化.pdf

1、河南師范大學(xué)碩士學(xué)位論文小鼠子宮巨噬細(xì)胞的分離、鑒定及其在妊娠早期的變化姓名：宋興麗申請學(xué)位級別：碩士專業(yè)：動物學(xué)指導(dǎo)教師：張順利20060501AbstIact0bjective：Toestablishtheis01ationandidcntificationmethodsofmacrophagcinuterine，andtostudythechangesofamountofmouseutedncmacrophageaIldtheex

2、pressionofA(、PandAKPdurillgearlypregnancy，andtostudytheroleofmacrophageinthecourseofe盯lypre印aIlcyMethods：MatedalsdiVidedinto咐ogmups：coⅡtml掣oupandoperat‘ed伊oupIsolatememacrophagebythemethodSofdigestionaIldhcterotypiclymph

3、o‘cyte，Macrophageswereisolateda11dpurifiedbyanchoringcIlltiVationTostudythech柚geofmacrophagebyttlemethodsofimmunochemistryandcnzymoc”ochemistryResuIt：(1)neisolationalldidentificationme血ods0fmouseutednemacrophageWhichthis

4、experimentfoundweresimple，ec0ⅡomicandconVenient(2)neresunsofimmuⅡo’chemistryshOwedthatthepositjvemacrophagenot0nlychangethe鋤ountbutalsothedistributionIncontrolgroup，mostlymacrophagearelieinendometrium_buttllegmupofD2，mos

5、tlylieinmyome仃iumandperimetrium，(P001)andmemacrophageinend0metri哪ofD3isthcleastP001)(3)withthetimeofpre印aIlcy90esby，mecontentofACPascendesbut硪盯D3，itdescentesComparedwiththenornlalgrOup，meIesuhsofenzymocytochemistryshowth

6、e柚殂contentofoperated舒oupisheigll，D4ispeakConclusioⅡ：T11emethOdsOfmacrophageisolationandidentificationinthisexperimentweresimpleaIldconveⅡient，wllichwillbeabasisforfurtherresearchofuteriIIemacrophageandpre鱸ancymatemo—feta

7、liIn皿unotoleranceThemacrophageplaysmeimportantmlesinearlyprc印ancymerearcmanychaIIgesofACPandAKPduringeadypregnancyMacrophagefunctionhassomerelationsllipwitllexprcssiOnOfACPandAKPKeywordsmacrophageute血ecDl4acidphosphatase