二甲胂酸誘導(dǎo)胃癌細(xì)胞SGC-7901凋亡的研究.pdf

1、Y85G571蠶佩天誓碩士學(xué)位論文(2006屆)二甲胂酸誘導(dǎo)胃癌細(xì)胞SGC7901凋亡的研究S；udyonlhcApoptosisofGastricCancerSGC一7901CellsInducedbyDimethy／arshficAcid硼究牛姓名——————』丑業(yè)包———一指導(dǎo)教師姓名—————皇點(diǎn)盅———一專幢名稱皇塹擔(dān)生當(dāng)金生鹽生研究方向————貯生魚盟煎主—一淪文提空日期————』她虹生王且—一蘭坐型!竺!皇!塑2￡!!!!

2、!!!魚苧型!曼竺!!!蘭璺蘭：!!Q!曼型堅(jiān)!!!!!塑!蘭望!竺!坐!竺!!!!苧!翌壘壘!!翌竺StudyonTheapoptosisofGastricCancerSGC7901CellsInducedbyDimethylarsinicacidAbstractobjective：Toobservetheeffectsofdimethylarsinicacid(DMAA)ongastriccfl_n_cerSGC7901cellsa

3、ndinvestigatesitsmechanismprimarilyMethods：AftercoculturingSGC7901cells、析tlldifferentconcentrationsofDMAA，theinhibitoryratio，morphologicalchangesandbiologicalchangesweredetectedbYMTTassayLaserScanningConfocalMicroscopyan

4、dDNAgelelectrophoresis，respectivelyResults：TheeffectiveconcentrationsofDMAAinducingapoptosisarefromO25mmol／Lto50mmol／LMorphologicalchangesincludingcondensationofcellularchromatinandfragmentationofnuclearandapoptoticbodie

5、s、Ⅳe咒observedFragmentationofintemucleosomalDNAwasveryapparentonelectrophoreticpatternsatthisconcentrations，bothofwhicharetypicalfeaturesofapoptosis，whileathighconcentrations(10mmol／L)DMAAcausedmainlynecrosisAtthesalD_eti

6、me，MTTassayindicatesthatthecytoactivationdecreasescorrespondinglywitlltheconcentrationsincreaseConclusions：neresultssuggestthatDMAAinducesapoptosisofSGC7901cellsatlowconcentrations，whileathighconcentrationsDMAAmainlycaus