桂枝養(yǎng)心方提取物對缺氧h9c2心肌細胞sdh、cpt1、atp影響的研究

1、分類號：密級：碩士研究生學(xué)位論文論文題目（中文）桂枝養(yǎng)心方提取物對缺氧H9C2心肌細胞SDH、CPT1、ATP影響的研究論文題目（外文）ResearchontheeffctoftheGuizhiYangxinRecipeonSDH、CPT1ATPinmyocardialcellsofratswithhypoxia研究生姓名曹亞斌學(xué)科、專業(yè)中西醫(yī)結(jié)合臨床研究方向中西醫(yī)結(jié)合防治心血管疾病導(dǎo)師姓名、職稱鐘栩主任醫(yī)師論文工作起止年月2016年1

2、月至2017年3月論文提交日期2017年3月論文答辯日期2017年5月學(xué)位授予日期2017年6月萬方數(shù)據(jù)摘要..................................................................................................................................................................1Abs

3、tract..............................................................................................................................................................3縮略詞表....................................................

4、..................................................................................................5前言.......................................................................................................................

5、...........................................6實驗研究......................................................................................................................................................81材料..................

6、......................................................................................................................................81.1實驗細胞系.............................................................................

7、...................................................81.2實驗用藥....................................................................................................................................81.3實驗主要儀器...................

8、.........................................................................................................81.4實驗試劑...........................................................................................................

9、.........................91.5主要試劑配制............................................................................................................................92實驗方法.......................................................

10、......................................................................................102.1H9C2心肌細胞培養(yǎng).................................................................................................................102.2中

11、藥制備..................................................................................................................................102.3心肌細胞造模............................................................................

12、..............................................112.4心肌細胞用藥濃度及藥物干預(yù)時間選擇..............................................................................112.5心肌細胞分組及給藥時間..........................................................

13、............................................122.6檢測指標...................................................................................................................................122.6.1心肌細胞形態(tài)學(xué)觀察....................

14、...............................................................................122.6.2心肌細胞琥珀酸脫氫酶（SDH）活性測定...............................................................132.6.3心肌細胞肉堿脂酰轉(zhuǎn)移酶（CPT1）活性測定........................

15、................................132.6.4心肌細胞三磷酸腺苷（ATP）含量測定....................................................................142.7數(shù)據(jù)統(tǒng)計......................................................................................

16、.............................................163結(jié)果......................................................................................................................................................163.1心肌細胞形態(tài).........

17、.................................................................................................................163.2各組心肌細胞SDH活性的比較........................................................................................

18、....163.3各組心肌細胞CPT1活性的比較..........................................................................................183.4各組心肌細胞ATP含量的比較.......................................................................................

19、......19討論................................................................................................................................................................201心肌細胞能量代謝.......................................

20、......................................................................................202藥物對缺氧心肌細胞代謝酶活性及ATP含量的影響....................................................................212.1藥物對缺氧心肌細胞SDH的影響....................

21、....................................................................212.2藥物對缺氧心肌細胞CPT1的影響......................................................................................222.3藥物對缺氧心肌細胞ATP的影響.........................

22、................................................................233桂枝養(yǎng)心方組方及現(xiàn)代研究...............................................................................................................23結(jié)語..........................

23、......................................................................................................................................261結(jié)論.................................................................................

24、.....................................................................262體會與展望.........................................................................................................................................26參考文獻

25、..................................................................................................................................................27附錄......................................................................