24494.不同分子大小的液相物質(zhì)的內(nèi)吞途徑及sec10在哺乳動物細(xì)胞胞吞中的功能探討

1、華中科技大學(xué)碩士學(xué)位論文不同分子大小的液相物質(zhì)的內(nèi)吞途徑及SEC10在哺乳動物細(xì)胞胞吞中的功能探討姓名：萬滔申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：張蓉穎20110121華中科技大學(xué)碩士學(xué)位論文IIAbstract?Endocytosisplaysakeyroleincellularactivities.Whentheprocessofendocytosisisblockedthecargoistransptedtothe

2、wrongdestinationitwillleadtovariousdiseases.Muchwkhasbeenfocusedonclathrinmediatedendocytosis(CME)whichisutilizedbytheinternalizationofspecificmembranereceptsextracellularnutritionalmolecules.Theclassicdefinitionfmacropi

3、nocytosisisthebulknoniveuptakeofextracellularfluidmacropinosomesfmedduringthisprocessvaryinsizefrom0.2to5?mindiameterduetotheabsenceoftheinductionbytheparticlesingestedthenucleationofcoatsfmedontheplasmamembranes.Thecapa

4、citytodissectthecontributionofmacropinocytosistocellularprocesseshasbeenhamperedbyalackofuniquemolecularmarkersdefiningfeatures.Inthisstudyweinvestigatedtheendocytosisoftwodifferentlysizedfluescencetaggeddexrans(10kDa70k

5、Darespectively)twowidelyusedfluidphasemolecules.Ourstudiessuggestedthatdynamindependentmicropinocytosis–independentmacropinocytosisarerequiredfsmallsizedfluidphasemolecules.Wheneitheroneisblockedtheotherwouldbeupregulate

6、d.Yetflargesizedfluidphasemoleculesmacropinocytosisisthepredominantway.Solargesizedfluidphasemoleculeswillbethebettertracerfmacropinocytosis.Exocytosisisanotherimptantphysiologicalprocess.Theexocystcomplexisconsideredtom

7、ediatethetetheringofvesiclestothemembranepritofusion.Exocystisamultimericproteincomplexhighlyconservedfromyeasttomammals.Howeverseveralrecentstudiessuggestedthatexocystmayhavearoleintheendocyticpathway.UsingRNAitechnique

8、westudiedtheroleofSEC10inmammaliancellswhichisonecomponentofexocyst.WefoundthatSEC10participatedinclathrinmediatedendocytosispathwayprobablybyaneffectontheearlierstageofpostendocytictransptofmoleculeswhichindirectlyledto

9、theimpairmentofendocytosis.Keywds:EndocytosisClathrinMacropinocytosisDextranDynaminSEC10ThiswkwassupptedbygrantsfromtheMajResearchplanfromtheMinistryofScienceTechnologyofChina(No.2007CB914200)theNationalScienceFoundation