26567.產(chǎn)腈水解酶菌株的篩選及其培養(yǎng)條件優(yōu)化

1、河南大學碩士學位論文產(chǎn)腈水解酶菌株的篩選及其培養(yǎng)條件優(yōu)化姓名：舒志愚申請學位級別：碩士專業(yè)：微生物學指導(dǎo)教師：楊生玉20100501產(chǎn)腈水解酶菌株的篩選及其培養(yǎng)條件優(yōu)化AbstractDuringtIleprocessofpmducingac巧licacidbyconVertingac巧loni仃ileusingamicrobialmethodthenitrilaSeplayedacriticalroleThee塌ciencyofcon

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5、nsf0rdeteminateacrylicacidw嬲雒follows：PEG一20McapillaD，c01umn(30m032mmid，025pm，)，tempe仡：turePmg舳ming(theinitialtemperatIlreof100℃，aRer1minriset0200℃by70℃／min)，thedetectorandVaporizationchambertemperaturewere230℃鋤d2lO℃，ca耐e

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9、roducingstmin0ruSewittlotherninogensourceScreeningnitrilaseproducingSn面nsbye耐chmentculture，t11roughadd∞巧lonitrileimo廿leb2LsicmediumStepbyStep，oruSingac拶lonitrile弱thes01eNsource／Csource，w1lincreaSedt0theconcennationofac拶l

10、oni仃訂e廳om001％t02％，incre叢einni仃ilaSe巾roducingSnainsinthemedium，卸dimproVestminS’toxictoleranceUsingas唿ine州chedandscreened舶mthesoil，06l4P，w笛the硎ginals汀ajn1n硼iationbyultraViolet砌iationtoscreeningmu鋤tswimhi曲erni仃ol勰e觚tiVi劬tll