小麥組蛋白修飾酶基因及DNA甲基轉(zhuǎn)移酶基因的分離及鑒定.pdf

1、中國農(nóng)業(yè)大學(xué)博士學(xué)位論文小麥組蛋白修飾酶基因及DNA甲基轉(zhuǎn)移酶基因的分離及鑒定姓名：戴艷申請學(xué)位級別：博士專業(yè)：作物遺傳育種指導(dǎo)教師：孫其信倪中福20050401AbstractEpigenelicregulationisamajoraspectofgenecontrolbywhichheritablechangesingeneexpressionOCCUrwithoutanalterationinDNAsequence，whichma

2、inlyincludeshistonecode，DNAmethylation，RNAinterferenceandgenomieimprintingThestudyofepigenticsinwheatlaggedbehindInthisarticle，genesencodinghistonemodificationenzymeandDNAmethltransferasewereisolatedusingdegenerateprimer

3、PCRand／nsilicocloningThemainresultsareasfollows：1)TwohistoneacetyltransferasegenesandfourhistonedeacetylasegeneswhichbelongedtodifferenttypeswereisolatedusinginsiHcocloningandRTPCRmethod，designatedTaHATI2，TaHDI一4respecti

4、velyAminoacidsequenceanalysissuggestedthattheyallcontainedtypicalstmctruecharacteristicsofhistoneacetyltransferaseanddeacetylaseSubeellularlocalizationindicatedthatTaHAT／proteinbecameconcentratedinthenucleusandTaHD3displ

5、ayednuclearcytoplasmicdistributionToourknowledge，nogenesencodinghistoneacetyltransferaseanddeacylasehasbeenreportedinwheat2)NineSETdomainproteingenefragmentswereobtainedbydegenerateprimerPCRPhylogeneticanalysissuggestedt

6、hattheybelongedtoSU(VAR)39classSETdomainproteinInaddition，twoSETdomainproteingeneswereobtainedusinginsilicocloningandRTPCRmethod，named砌齲刀2respectivelyPhylogeneticanalysisindicatedthattheybelongedtoASHandSU(VAR)39classSET

7、domainproteinrespectively3)WeisolatedfourwheateDNAfragmentsandoneeDNAwithopenreadingframeencodingDNAmethyltransferaseanddesignatedTaMETl，TaMET2a，TaMET2b，TaCMTandTaMET3，respectivelyBLASTsearchesandphylogeneticanalysissugg

8、estedthat5cDNAsbelongedtofourclasses(Dnmtl，Dnmt2，CMTandDnmt3)ofDNAmethyltransferasegenesTaMET2aencodedaproteinof376aaandcontainedeightoftenconservedmotifscharacteristicofDNAmethyltransferaseGenomicsequenceofTaMET2awasobt

9、ainedandfoundtocontaintenintronsandelevenexons4)Expressionanalysisofthesegenesobtainedinthisarticlerevealedthattheywereexpressedindevelopingseed，duringgerminationandvariousvegetativetissues，butinquitedifferentabundanceIt

10、wasinterestingtonotethatTaMETlandTaMET3involvedinmaintenancemethylationanddenOVOmethylationwereclearlydetectedindryseedsandothergeneshadnotbeendetectedMoreoverdifferentialexpressionpatternsofthesegenesWereobservedbetween

11、wheathybridanditsparentsinleafstemandrootofjointingstage，somewereupregulatedwhilesomeothersweredownregulatedinhybrid5)OverexpressionofTaHAT／andTaHD3genesinArabidopsissuggestedthattheywerebothassociatedwithvariousdevelopm

12、entalabnormalities，mainlyincludingleafandflowerdefectwhichsuggestedthat如J刪ZYandTaHD3maybeinvolvedinageneralregulatorymechanismresponsibleforplantplasticityandvariationinnatureThisisafirstreportaboutfunctionstudyofhistone