黑穗醋栗葉片中黃酮類化合物的提取、分離純化及生物活性的研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文黑穗醋栗葉片中黃酮類化合物的提取、分離純化及生物活性的研究姓名：張志昆申請學(xué)位級別：碩士專業(yè)：園藝學(xué)；果樹學(xué)指導(dǎo)教師：于澤源20120620AbstractStudiesonExtraction，PurificationandBioactivitiesofFla，VonoidsinBlackCurral址LeavesAbstractBlackcu玳mtcommonlyl(nownaSblackbeanfⅢit’i

2、saspecialkindofsmanberriesincoldareaHeilon萄iang，theleaVesarerichinflavonoidsThestudychoosetheleaVesofblackcun‘a(chǎn)llt弱thetlleexperiment“materials，IntheresearchofnaVonoidsbaSedonthebestextractionprocess，systematicallyresearc

3、hflaVonoidsseparation，purification，identificatiOnaIldbiologicalactiVi劬expectt0pmVidesupportforblackcurraIltindustrialproductionInextractionprocessusingresponsesu噸lcemethodoptimizedultr鷦oniceXtractionconditions：detenlline

4、theoptimumtheoreticalconditions：uItraSonictime30min，ultr硒onicpower517W，liquidtomaterialratio15。2：lmL儋，navonoidseXtracteduIlderthisconditionStheoreticalpredictedValueis46916mg儋，actualoperatingconditions：ultrasonictime30mi

5、n，ultraSonicpower520W，liquidtomaterialratio152：1mL儋，navonoidseXtractedundertheseconditionstlle鋤ountofupto45891mg儋，isVe叫closetothepredictedValue46916mg信studypurificationthrou曲macroporousresinaSadso叩tionmaterial，throughthe

6、stalicexperimentcomparesixmacroporousresineffectofadsorptionanddesorptionnavonoidsoftheblackcu刪ltleaves，chosethreekindsofmacroporousresin：NKA9、D4020、D1011，mrou曲theadsorptionanddesorptiondyn帥icexperiment，detemineNKA9aSexp

7、erimentalresinpurifiedthenavonoidsofblackcurralltleavesTheoptimumconditionsofNKA9dyn鋤icadsorptionaIlddesorptiontheflaVonoidsofblackcurrantleaVesis：at25℃，theadsorptionrate1mL／衲in(concentrationO9311m咖L)，adsorptiontime1h，th

8、es鋤plevolume60mL，anhydrousethanolaSeluant，elutionflowratelmL／min，elutiontime100min，elutionvolume1OOmL，undertheseconditions，thepurityofblackcunantleavesflaVonoidsisupto4015％，comparedtheformer(withoutp嘣fication)(953％)，thep

9、urityenhancedmorethan4timesTheseshowthatNKA9macroporousresinpurineswellIntheseparationprocess，meSephadexLH20cOlumnwaSappliedtoseparationOfthepurifiednaVonoids，anhydrousmethanolwaSchosenaSelution，solventelutionnowratewas1

10、0mL／min，衄ou曲theHPLCanalysis(cIlromato伊印hyconditionswereaS：column：Agilent一1l00C18c01umn；mobilephase：methaJlol：water(50：50)；nowrate：10mL／min；detection：Agilent—G13l4AUVdetector；detectionwaVelen舀h：360nrn；temperature：roomtemp