麝香烏龍丸論文麝香烏龍丸對(duì)aa大鼠滑膜襯里層病理形態(tài)及bfgf、tgf-β1表達(dá)的影響

1、麝香烏龍丸論文：麝香烏龍丸對(duì)麝香烏龍丸論文：麝香烏龍丸對(duì)AAAA大鼠滑膜襯里層病理形大鼠滑膜襯里層病理形態(tài)及態(tài)及bFGFbFGF、TGFβ1TGFβ1表達(dá)的影響表達(dá)的影響【中文摘要】觀察麝香烏龍丸對(duì)佐劑關(guān)節(jié)炎(AA)大鼠滑膜襯里層病理形態(tài)及堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)、轉(zhuǎn)化生長(zhǎng)因子β1(TGFβ1)表達(dá)水平的影響探討麝香烏龍丸治療類風(fēng)濕關(guān)節(jié)炎(rheumatoidarthritisRA)是否與調(diào)節(jié)bFGF和TGFβ1水平有關(guān)。方法

2、將健康Wistar大鼠30只隨機(jī)分為正常組(A組)、模型組(B組)、麝香烏龍丸組(C組)采用弗氏完全佐劑法建立模型麝香烏龍丸灌ig1周后HE染色、光鏡下觀察各組滑膜襯里層病理變化、襯里層滑膜細(xì)胞層數(shù)免疫組織化學(xué)法檢測(cè)各組滑膜襯里層bFGF、TGFβ1表達(dá)。結(jié)果1滑膜襯里層病理形態(tài)觀察:A組大鼠滑膜襯里層由12層扁平型或低立方形上皮樣滑膜細(xì)胞組成且部分不連續(xù)滑膜組織無(wú)肥厚、充血B組滑膜襯里層增生到48層呈乳頭樣或柵欄樣突起細(xì)胞呈卵圓形排列

3、紊亂體積增大滑膜組織肥厚、充血水腫形成許多絨毛狀突起突向關(guān)節(jié)或侵入軟骨及骨質(zhì)C組襯里層數(shù)較B組減少滑膜細(xì)胞輕度增生滑膜組織充血水腫減輕。2滑膜襯里層細(xì)胞層數(shù)及bFGF、TGFβ1測(cè)定結(jié)果比較2.1滑膜襯里層層數(shù)B組、C組與A組比較層數(shù)低于A組差異有統(tǒng)計(jì)學(xué)意義(P0.01)C組與B組比較層數(shù)低于B組差異有統(tǒng)計(jì)學(xué)意義(P0.05)。實(shí)驗(yàn)結(jié)果表明麝香烏龍丸能降低滑膜襯里層細(xì)胞層數(shù)減輕滑膜細(xì)胞增生。2.2bFGF表達(dá)水平B組、C組與A組比較bF

4、GF表達(dá)高于A組差異有統(tǒng)layersynovialliningcelllayersexpressionofbFGFTGFβ1wereobserved.Results1.Theobservationofsynoviallininglayerpathologicalfm:GroupA:rats’synoviallininglayerbuildingupby12layerflattypelowsetsquareepithelialappear

5、anceofsynovialcellssomeofdiscontinuoussynovialganizationwithouthypertrophycongestion.GroupB:hyperplasiaofthesynovialliningto4to8layersinsamplethefencepapillarylayersampleswelledthecellsareovoidarrangingdisderlyincreasing

6、insizeSynovialganizationishypertrophycongestionoedemafmingmanyfluffquiltedaxontojointsinvadescartilagebone.ThelininglayeringroupCbelowsitingroupBsynovialcellsmildhyperplasiasynovialganizationhyperemiaedemaease.2.Resultco

7、mparisonofcelllayersofSynoviallininglayerbFGFTGFβ1determination:2.1SynoviallininglayersingroupBCcomparedwithgroupAlayersbelowingroupAastatisticallysignificantdifference(P0.01)GroupCcomparedwithgroupBlayerbelowtheBgroupas