山姜素對U87人腦膠質(zhì)瘤細胞的促凋亡作用及其機制研究.pdf

1、分類號：R651密級：公開UDC：610學校代碼：11065碩士專業(yè)學位論文山姜素對U87人腦膠質(zhì)瘤細胞的促凋亡作用及其機制研究王文來指導教師姚維成教授學位類別臨床醫(yī)學碩士專業(yè)領域外科學（神經(jīng)外）答辯日期20170519ⅡThePromotionalApoptosisAffectMechanismofAlpiinonU87HumanGliomaCells.AbstractBackgroundpurpose:Gliomaisoneofth

2、emostcommonintracranialmalignanttumsthembidityisaboutmethanhalfofallintracranialtumincidences.Thegliomaoflowdifferentiationrapidproliferationstronginvasivenessthanothertumjustonthesurgicaltreatmentisstillcannotbetotallyr

3、emovedaftersurgicalremovalofthecomprehensivetreatmentisstillcurrentlyrecommendedtreatmentplan.Alpiinisakindofnaturalcompoundsthatcaninhibittheproliferationofgliomacellsinvasionitisproventhatitpossessedtheapoptosisinducin

4、geffect.ThispapermainlyresearchonthedifferentexpressionofbaxBcl–2CaspasePARPonhumanbraingliomacellsU87.itsmolecularmechanismofpromotingapoptosis.WhileAlpiinbringsnewhopefgliomatreatment.Methods:Cultivatethehumanbrainglio

5、macellsU87invitroDetecttheproliferationinhibitionofalpiintoU87cellbyMTTassayDetectthedifferentexpressionofbaxBcl–2CaspasePARPbywesternblottingFlowcytometryanalysisthedifferenceofcellcycleafterdosingprocessedU87cellCellsc

6、ratchexperimentsshowedthemigrationabilityofdosingprocessedU87cellwassignificantlyinhibited.Results:AlpiinhasobviousinhibityeffectinadosedependentmanneronU87cellproliferationinvitrotheenzymeismeasuredat490nmbythevalueofth

7、e“OD“whichcanreflectthenumberoflivingcells.AlpiinongliomaU87cellproliferationhassignificantinhibityeffectastheconcentrationofrisingU87cellsurvivalratewillgraduallydeclinepresentsthetypicalconcentrationdependencerelationt

8、heIC50of7.122μgmlThewesternblottingshowedthatafterdosageofIC50alpiinconductedonU87celltheexpressionofbaxCaspaseissignificantlyhigherthanthecontrolgrouptheexpressionofBcl2PARPisinferitothecontrolgroupwhichpromptedthatalpi

9、incanpromotecellapoptosisofU87FlowcytometryanalysisshowedthatthecellcycleafterdosingprocessedU87cellisdifferentthanpreviousthedruggroupofU87cellsinG1phaseretardationTheapoptosisofalpiinconductedU87cellincreaseditwereconc

10、entrationdependent.Conclusion:AlpiincaninhibittheproliferationofhumanbraingliomaU87cellinvitrocultureinducedifferentexpressionofapoptosisrelatedgenewhichhassignificanteffectonpromotingapoptosisinhibitU87cellmigration.Cur

11、rentunderstingofcellapoptosishoweverbelievesthatcellsapoptosisisaverycomplicatedbymultiplegeneregulationtheprocessofalpiinintheprocessofhumangliomaU87cellsapoptosisinductionwhetherthereareothergenesinvolvedininwhatwaytop