根癌農(nóng)桿菌介導(dǎo)的納豆激酶基因在番茄中的轉(zhuǎn)化與表達.pdf

1、薩酬學號研究類型⑩愿；應(yīng)用研究』匕Z鬻鬢鬈攀攀譽7窿蓄震饕碩士學位論文學科門類學科專業(yè)屬赫赫擴攀黲罐讖然赫理學生物化學與分子生物學指導(dǎo)教師誓警謦苧苧研究生論文題目袁琳根癌農(nóng)桿菌介導(dǎo)的納豆激酶基因在番茄中的轉(zhuǎn)化與表達答辯白期：2∞6年5月21日薌臻◆瓣AbstractNattokitlase(NK)isafi陸lol州ce11zymethatisex位廊d矗Dmnano(a廿觚n伽Ialfc加entedfoodi11J印an)In脅ith

2、as伽臣丁macicpr叩er哆ofproteaseCompared謝m也ethromb01州cagelltssuchasurokinaseandstreptokmaseNa_ctokiIlaseisfaster，easiertobeabsorbedbybodybecauseofitslowmolecularweight，蛆dhasmoredirect趾dmorcpersistente玨bcton岫bosisSona牡okinaseca

3、IlbedevelopedasaⅡew丘七血01如cmediciⅡeInt11isrese缸ch，tomatopl觚ts謝Ilbe訂姐sfomedtoexpressnattol【iII嬲egenePeopleeatt11e仃aIlsge曲ctom砒。髓dneednottocookit，mean州lemeycanget也enattokinaseThefonowingw日廈khasbeendone：1Them：andpronksequenc

4、eswercclonedintotransf酹VectorpCIAMBIA2300，and也erecombinaⅡt衄sposonpl船耐dpC啪【A230011klwaspCIAMBIA2300如obta：medrespectiVelyThen11khaSa35s一5’∞quenceas也epromoterihmeups虹e鋤ofit；anos3’seq咖ceasthetemlillatorin也edownstre咖ofit2TraI

5、lsferthepCI舢佃IA2300畸ll【1w∞pC【l心仍IA2300一nk2into49陽6口c把一刪f甜聊岜廊c招，坫EHAl05Andwegetme49，D6ac招，mmf蜊電加把mEHAl05nklandEHAl05nk23Hypocotylewerecut舶m‘xifen3’(Lycopersiconesclll咖)，曲clllatcd謝mA伊0bac涮umtuInefhcicnsEHAl05contBiIlingnkg

6、eⅡePlamswereregeneraledonselectivemedia謝tll艮lnamysin111epresenceoftlle11kgene、vasco曲medbyPCRinpl鋤tssurviviI培sectionsR1’PCRresunsShowedtllat11l【gene(1】kaIldpro喲b吖ebeneexpres∞datthetranscIipitionleVel4Proteine)(仃acted筋m也e廿

7、ansgemicplam’slevel，analyzedbySDSPAGETheresultsuggestsme衄lsgenticp1鋤t也atjustcontam11ksequencec趾expressmeNK，bycollS竹act，me打aIlsgenticplant也atcontaillprodkc髓note)4)resstheNKKeywords：nanokinasegene(nkgene)to】nato，ge玎etic仃al