大腸桿菌高價鐵腸桿菌素受體蛋白FepA表面抗原決定簇基因的克隆及原核表達(dá).pdf

1、ClassifiedIndex:U.D.C:DissertationfortheMasterDegreeinEngineeringCLONEANDPROKARY.OTICEXPRESSIONOFSUR衛(wèi)ACEEPITOPEGENEINTHEFERRICENTEROBACTINRECEPTORPROTEINFEPAOFESCHERICHIACOLICandidate:SuPervisor:AeademicDegreeAPPliedfor:

2、SPeeiality:DateoforalExamination:University:認(rèn)厄ng腸Prof.LiuNingMasterofEngineeringFoodScienceJune，2009NortheastAgriculturalUniversity東北農(nóng)業(yè)人學(xué)工學(xué)碩卜學(xué)位論文3.3.3重組質(zhì)粒的鑒定……，............................................................

3、.................................……313.4融合蛋白在大腸桿菌中誘導(dǎo)表達(dá)的初步研究............................................................……333.4.1融合蛋白的誘導(dǎo)表達(dá)....................................................……，............................

4、.......……333.4.2融合蛋白表達(dá)形式的鑒定....................................................................................……344討論....................................……‘.…、.……、....……，.…、、……、......……、二、.…，.....……、、、...............……3

5、54.1目的基因的克隆....……，.................................................................................................……354，2表面抗原決定簇基因的選擇......................................................................................

6、..……354.3TA克隆方法的應(yīng)用.................................................……，.............................................……364.4原核表達(dá)載體的選擇...............................................……，.......................................

7、.......……364.5融合蛋白的誘導(dǎo)表達(dá)及鑒定.……、.…‘.、……，.............................................................……365結(jié)論..................................................................................................................

8、....................……38致謝................................................................................……，……，......................................……39參考文獻..............................................................

9、................................................................……，…40附錄...............................................................……，....................................................................……45攻讀碩士期間發(fā)表的學(xué)