原發(fā)性肝癌患者外周血Treg、MDC、PDC細(xì)胞水平變化及意義.pdf

1、周宏華原發(fā)性肝癌患者外周血Treg、MDC、PDC細(xì)胞水平變化及意義原發(fā)性肝癌患者外周血Treg、MDC、PDC細(xì)胞水平變化及意義中文摘要目的：檢測(cè)乙肝相關(guān)性原發(fā)性肝癌(Primaryhepaticcarcinoma，PHC)患者外周血調(diào)節(jié)性T細(xì)胞(regulatoryTcells，Treg)、漿細(xì)胞樣樹(shù)突狀細(xì)胞(plasmacytoiddendriticcell，PDC)2及髓樣樹(shù)突狀細(xì)胞(myeloiddendriticcell，M

2、DC)的表達(dá)，初步分析探討PHC患者外周血Treg、MDC、PDC細(xì)胞水平變化及意義。方法：收集江蘇省蘇北人民醫(yī)院肝脾內(nèi)科乙肝相關(guān)性PHC合并肝硬化患者30例(I組)、乙肝肝硬化患者16例(11組)、健康體檢者20例(ill組)外周血標(biāo)本，采用流式細(xì)胞術(shù)檢測(cè)PHC合并肝硬化患者(I組)、乙肝肝硬化患者(II組)、健康體檢者(Ⅲ組)外周血Treg、MDC、PDC細(xì)胞百分比，結(jié)合患者資料初步分析其意義：(1)三組外周血Treg、MDC、PD

3、C細(xì)胞百分比表達(dá)；(2)PHC組Treg細(xì)胞與MDC、PDC細(xì)胞百分比表達(dá)水平相關(guān)性：(3)PHC組腫瘤直徑與T／eg、MDC、PDC細(xì)胞百分比表達(dá)水平相關(guān)性：(4)PHC組患者HBVDNA載量與Treg、MDC、PDC細(xì)胞百分比表達(dá)水平相關(guān)性。結(jié)果：l、三組外周血中Treg細(xì)胞百分比檢測(cè)：Treg細(xì)胞在PHC組(856士187)％高于肝硬化組(6。77j：239)％和對(duì)照組(422土124)％，差異均有統(tǒng)計(jì)學(xué)意義(PO05)。3、三組

4、外周血中PDC細(xì)胞百分比檢測(cè)：PDC細(xì)胞在PHC組(O13士007)％低于對(duì)照組(034=1009)％、肝硬化組(017士007)％低丁對(duì)照組、PHC組低于肝硬化組，差異有統(tǒng)計(jì)學(xué)意義(尸005；，=033，P005)。5、PHC組腫瘤直徑與Treg、MDC、PDC細(xì)胞亞群百分比相關(guān)性：腫瘤直徑與Treg細(xì)胞呈顯著正相關(guān)(r=0992，P005；，=0289，PO05)。6、PHC組HBVDNA與Treg、MDC、PDC細(xì)胞亞群百分比相關(guān)

5、性：HBVDNA與Treg、MDC、PDC細(xì)胞均無(wú)顯著相關(guān)性(r=O053，P005：r=0423，P005；r=0238，P005)。結(jié)論：TheexpressionandsignificanceoftheTreg、MDC，PDCcellsintheperipheralbloodofpatientswithprimaryhepaticcarcinomaABSTRACTObjective：Toinvestigatetheproporti

6、onofperipheralbloodregulatoryTcells(Treg)，Myeloiddendriticcell(MDC)，Plasmacytoiddendriticcell(PDC)cellsinthepatientswithprimaryhepaticcarcinoma(PHC)andinfectedwithhepatitisBvirusffmv)，preliminaryanalysistheprobableroleof

7、Treg，MDC，PDCcellsinthepathogenesisofprimaryhepaticcarcinomaMethods：sixteenpatientswithlivercirrhosis，thirtypatientswithcirrhosisprimaryhepaticcarcinomawereenrolledinthestudy，allofthepatientswereinfectedwithHBVtwentyhealt

8、hyvolunteerswereselectedasthecontrolsTheperipheralbloodspecimenswerecollected，andtheproportionsofTreg，MDCandPDCcellsweredetectedbyflowcytomet9(FCM)Theclinicalsignificanceofchangesconcerningtheclinicaldatewerepreliminaril

9、ydiscussedTheproportionofperipheralbloodTreg，MDCandPDCcellsamongthreegroupswerediscussedThecorrelationofTregcellsandMDC，PDCcellsinPHCpatientswereanalyzedThecorrelationofTreg，MDCandPDCandserumHBVDNAlevels，tumordiameterinP

10、HCpatientswerealsoanalyzedrespectivelyResults：1、ThelevelsofTregcellsintheperipheralbloodofPHCpatients，livercirrhosispatientsandhealthycontrols：ThepercentageofTregcellsinPHCpatients[(856士187)％】wassignificantlyincreasedtha

11、ninlivercirrhosispatients[(677239)％】andhealthycontrols[(422124)％】護(hù)005)3、ThelevelsofPDCcellsintheperipheralbloodofPHCpatients，livercirrhosispatientsandhealthycontrols：ThepercentageofPDCcellsinPHCpatients[(013士007)％】wassig