高產幾丁質酶菌株的篩選與CK1-3菌株幾丁質酶的純化與鑒定.pdf

1、江西師范大學碩士學位論文高產幾丁質酶菌株的篩選與CK13菌株幾丁質酶的純化與鑒定姓名：羅玉彬申請學位級別：碩士專業(yè)：生態(tài)學指導教師：王曼瑩201105AbstractChitinisoneofthemostabundantorganicsintheworldEveryyearthesyntheticarnountofchitincallreachmorethantenbillionthestockpileofchitincomessec

2、ondaftercellulose，it’SaninexhaustiblerenewablenaturalresourcesintheWO訂dChitinasespecificallycallcatalysethereactionofhydrolyzingchitinintoNacetylglucosamine(GlcNac)，Watersolublechitinand(GlcNac)nStudyonscreeningchitinase

3、producingbacateriafrommicrobehaveevidentsciencevalue，andalsohasgreatpracticalsignificanceincleanerproductionandwi(1euseofnaturalchitinsuchasbiologicalcontrol，environmentalprotectionfoodandmedicalexploitationnlisexperimen

4、thaveextensivescreened、^，imthehighproducingchitinasesrtains，andhaveselectedouteightyninechitinaseproducingstrainswhichhavebeenusedascandidatestainsWealsoIsolatedanddepuratedthechitinaseofahighproducingchitinasestrainwhic

5、hnamedCKl3CKI一3iSasaccharomycetesbyprimaryidentificationWehaveoptimizethefermentationconditions’sofCKI3，andhavebeensurethattheoptimalcompositionsis：colloidalchitin80，peptone5，yeastextract5，K2聊P04O7，KH2P0403，MgS047H2005，F(xiàn)

6、eS047H20O01，znS04O01，PH68；ThcoptimalcultivationconditionsforCKt一3is：OriginalpHis68，inoculumconcentrationis14％(w／v)，250mLconicalflaskcontain50mLfermentationliquor30oCincubatingtemperatureandshakingcultivationat180r／rainfo

7、r120hnlemaximalchitinaseactivityoffermentedliquidCanreach251I，／mI，WehaveestablishedtheIsolatedanddepuratedconditionofstrainCKI3’SfermentationliquorRefrigeratedcentrifugethefermentationliquorsedimentethesupernateby85％icee

8、thanol，centrifuge，dissolveinasmallamountofwaterthenwehavegottheroughchitinaseAfterpurifiedbySephadexG100，wehavegotthemacromolecularchitinaseimmediately硼1epurificationfoldis661，andyieldcoefficientis372％SDSPAGEcataphoresis