離子對-HPLC測定牛奶和雞肉中氟喹諾酮藥物殘留.pdf

1、河北大學(xué)碩士學(xué)位論文離子對HPLC測定牛奶和雞肉中氟喹諾酮藥物殘留姓名：秦麗娟申請學(xué)位級別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：呂運開201105AbstractIIAbstractSeparationanalysismethodsofveterinarydrugresiduesinfoodofanimaliginaredevelopedwhichisofimptancetocontrolfoodsafetyensurehumanhealth.

2、Inthispapertwonewmethodsofionpairreversephasehighperfmanceliquidchromatographywereestablishedfthedeterminationoffluoquinolonesresiduesinmilkchickentissue.Chromatographicconditionswereinvestigatededindetailafastaccuratese

3、nsitivemethodsofthemultiresiduesdetectionactualsamplepreparationweredeveloped.Themaincontentsasfollow:Chapterone:Thedangersoffluoquinoloneresiduesitsdetectionmethodswereintroducted.Theapplicationprogressoftheionpairreage

4、ntinhighperfmanceliquidchromatographywerereviewed.Chaptertwo:Anovelionpairreversephasehighperfmanceliquidchromatographymethodhasbeendevelopedvalidatedfthesimultaneousdeterminationoffivefluoquinolonesresiduesinmilk.Thestu

5、diedfluoquinoloneswereofloxacinciprofloxacinenrofloxacinnfloxacinsarafloxacin.ChromatographicseparationwascarriedoutonaVenusilXBPC18columninisocraticmodewith0.025molLH3PO4solutionadjustedtopH3.2with13mmolLtetrabutylammon

6、iumbroeacetonitrile(94:6vv)asmobilephase.Goodlinearityovertheinvestigatedconcentrationrangewasobservedwithmeanvaluesofcrelationcoefficientshigherthan0.9988falltheanalytesstudied.Thelimitofdetection(LOD)thelimitofquantifi

7、cation(LOQ)were4.3917.44gkg14.6458.14gkgrespectivelyThesevaluesareincompliancewithrequirementsfmonitingofmaximumresidueslevels(MRLs).Overallrecoveriesfromspikedmilksamplesrangedfrom80.7to96.92%withrelativestarddeviations

8、(RSD)lowerthan4.23%.Chapterthree:AsimplesensitivereliableionpairRPHPLCmethodhadbeendevelopedfthedeterminationofsixfluoquinolonesresiduesinchickentissue.AVenusilXBPC18column(150mm4.6mm5m)analyticalcolumnwasusedwithaneluti

9、ngsystemconsistingofamixtureof0.4molLcitricacid(pH=2.54)–acetonitrile–methanol(77:7:16%vv)operatingconditionwere:1mLminflowrate20Lvolumeinjectiondetectionat272nm.Thelinearconcentrationrangeofapplicationwas0.05~2gmLfsixco