結(jié)直腸癌患者血清IL-6水平及與其基因啟動子-174G→C多態(tài)性的關(guān)系.pdf

1、摘要目的門0g吉直腸癌患者血清白細胞介素“(IL6’水平及其臨床意義探討結(jié)直腸癌患者IL6啟動子一174GC多態(tài)性的存在及與血清IL6水平的關(guān)系?！傲艘灰环椒?用ELISA法測定結(jié)直腸癌患者血清IL6水平，電化學發(fā)光免疫分析法測定血清CEA水平，PCRRFLP法對結(jié)直腸癌患者進行IL6啟動子一174GC多態(tài)性分析。結(jié)果:1.結(jié)直腸癌患者血清IL6水平為30.02110.26ngml，顯著高于正常對照組的21.0912.81ngml(P0

2、.05).2.DukesD期患者血清IL6水平為35.88111.22ngml，顯著高于DukesBC期患者的26.95士7.72ngml和28.44士9.68ngml(P0.05)o肝轉(zhuǎn)移患者血清IL6水平為38.04士10.83ngml，顯著高于未發(fā)生肝轉(zhuǎn)移患者的28.4619.51ngml(P0.05)。患者血清IL6水平與性別、年齡及腫瘤位置無明顯相關(guān)。3.結(jié)直腸癌患者血清IL6和CEA水平的相關(guān)系數(shù)r=0.385(P0.05)

3、，兩者并無明顯相關(guān)。4.49例結(jié)直腸癌患者及正常對照均為正常純合子一174GG.結(jié)論:1.結(jié)直腸癌患者血清IL6水平顯著增高。產(chǎn)2.結(jié)直腸癌患者血清IL6水平與Dukes分期、肝轉(zhuǎn)移相關(guān)與患者性別、年齡、腫瘤位置及血清CEA水平無關(guān)。3.暫未發(fā)現(xiàn)結(jié)直腸癌患者中存在IL6啟動子一174GC突變。均為正常GG型。之蘭，一一TherelationshipbetweentheserumIL6levelsandthe一174GCpolymorph

4、ismoftheIL6genepromoterinpatientswithcolorectalcancerABSTRACTObjective:Toinvestigatesigmficanceoftheseruminterleukin6(IL6)concentrationsinpatientswithcolorectalcancer，detect一174GCpolymorphismofIL6genepromoterandstudyther

5、elationshipbetweenconcentrations.一174GCpolymorphismandtheserumIL6Methods:ELISAassaywasusedtodetecttheserumconcentrationofinterleukin6CEAwasexaminedbyelectrochemiluminescenceimmunoassay一174GCpolymorphismofIL6genepromotero

6、fpatientwithcolorectalcancerwasdetectedbyPCRRFLP.Result:I.TheserumIL6levelinpatientswithcolorectalcancerwas30.02110.26ngmlandwassignificantlyhigherthanthatinthehealthygroup(21.0912.81ngml)(P0.05)，2.SerumIL6levelinpatient