Mithramycin A對(duì)大腸癌細(xì)胞株骨橋蛋白OPN、轉(zhuǎn)錄因子Sp1表達(dá)影響的實(shí)驗(yàn)研究.pdf

1、福建醫(yī)科大學(xué)碩士學(xué)位論文MithramycinA對(duì)大腸癌細(xì)胞株骨橋蛋白OPN、轉(zhuǎn)錄因子Sp1表達(dá)影響的實(shí)驗(yàn)研究姓名：黃旭申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：病理學(xué)與病理生理學(xué)指導(dǎo)教師：張聲20081201TheexperimentalstudiesofeffectsofMithramycinAontheexpressionofosteopontin、SplinthecolonrectalcarcinomacellsABSTRACTobjective

2、：ToexploretheexpressionoftranscriptionfactorSplandosteopontin(OPN)inthecolorectalcarcinomacelllines(SW480andSW620)wi也differentmetastaticpotential，WetreatthelineswiththemithramycinA(MIT，aSplinhibitor)，andobservecellcultur

3、es’growth，exploretheinfluencesofOPNexpressionTheaimofthisstudyistodetecttheeffectsofSplandOPNonproliferationandmetastasisofthecolorectalcancerwhethertranscriptionfactorSplcouldbeaneffectivetherapeutictargetforhumancolore

4、ctalcancelMethods：TakenestablishedhumancolorectalcancercelllinesSW480andSW620invitroasresearchobject，RealtimePCR，Western—blotmethodsandcellularimmunofluorescencetechniquewereusedtodetecttheSpl、OPNexpressionatgeneleveland

5、proteinexpressionlevelbeforeandaftertreatmentwimMithramycinA，separatelyCellgrowthcurvewasusedtoassesstheinhibitoryeffectoftreatmentwitllMithramycinAoncellproliferationThemigrationpotentialofcancercells嘶thdifferenttreatme

6、ntswasanalyzedbythewoundhealingassayrespectivelyTheproductionofmatrixmetalloproteinases(MMPs)ofSW480andSW620celllinesweremeasuredbygelatinzymogrambeforeandaRertreatmentwithMithramycinAResults：RealtimePCRresultsshowedSW48

7、0(DukesBstage)andSW620(DukesCstage)hadSplandOPNexpression；Western—blotalsoshowedtheexpressionofSplandOPNproteinexistedinbothcelllinesThelevelofSplandOPNexpressionincelllineSW620WashigherthaninSW480(P005)；aftertreated、^，i

8、mMithramycinA(010肛M，)，thelowlevelofSplandOPNexpressioninbothlineswerevalidatedbyRealtimePCR，westernblottinganalysisandcellularimmunofluorescencetechnique(P005)；Cellgrowthcurveshowedtheproliferationdecreasedaftertreatment