外源纖維酶在反芻動(dòng)物胃腸道中主要作用部位和主要作用模式的研究.pdf

1、內(nèi)蒙古農(nóng)業(yè)大學(xué)博士學(xué)位論文外源纖維酶在反芻動(dòng)物胃腸道中主要作用部位和主要作用模式的研究姓名：呼和申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：動(dòng)物營養(yǎng)與飼料科學(xué)指導(dǎo)教師：嘎爾迪20080601EffectsofExogenousFibrolyticEnzymesonMainSitesandTheirModesinDigestiveTractofRuminantsAbstractThreehealthChineseHolsteinlactatingCOWSwi

2、thpermanentruminalcannulaswereselectedasexperimentalanimalsTheexperimentconsistedofthreeexperimentalperiods，eachcomprised20daysDuringeachexperimentalperiod，thedietofCOWSWassupplementedwith0(contr01)，lkgoffibrolyticenzyme

3、preparationIorlkgoffibrolyticenzymepreparationIIpertormeofdrymatter(DM)Thestudieswereasfollows：(DEflectofexogenousfibrolyticenzymesondigestionofnutrientsintheru／nensuchaseffectofralliesfermentation，fiberdegradationandmic

4、robialNflowtoduodenum②Effectofexogenousfibrolyticenzymesonrurflenmicrobialpophlationof蜘cows，suchasprotozoa、bacteria、fungiquantities；establishmentsofarelativequantificationofrealtirnePCRforUseinfibrolyticbacteriaandstudie

5、sontheeffectofexogenousfibrolyticenzymesonlllmenfibrolyticbacteria③Effectofexogenousfibrolyficenzymesondigestionandmatebolizationinda蛔COWS。Inanotherexperiment，6twoyearsoldInnerMongoliansemifinewoolsheep(bodyweight，2730kg

6、)，fitted、Ⅳi吐lpermanentcannulasintheBlmenandattheproximalduodenumandterminalileumweredividedinto2groupsof3inonefactorexperimentaldesignTlaedietofsheepwassupplemented晰t110(contr01)，lkgoffibrolyticenzymepreparationIpertorme

7、ofdrymatter(DM)Thestudieswereasfollows：@EstablishanassaymethodforUseinmeasurementoffibrolyticenzymesactivityindigestivetractofruminants②Effectofexogenousfibrolyticenzymesoncellulaseandxylanaseactivityinrumenandsmallintes

8、tineTheresultshowedasfollows：@Theexogenousfibrolyticenzymeswerebeneficioltumenfermentation；ThedigestionofDM、NDF、ADFinlumenandthemicrobialNflowtoduodenumwereincreasedsignificantlyaftersupplementingexogenousfibrolyticenzym