三血管結(jié)扎法制備兔后循環(huán)缺血模型的評價.pdf

1、新疆醫(yī)科大學(xué)碩士學(xué)位論文三血管結(jié)扎法制備兔后循環(huán)缺血模型的評價姓名：庾建英申請學(xué)位級別：碩士專業(yè)：神經(jīng)病學(xué)指導(dǎo)教師：朱沂2010-04新疆醫(yī)科大學(xué)醫(yī)學(xué)碩士學(xué)位論文 新疆醫(yī)科大學(xué)醫(yī)學(xué)碩士學(xué)位論文 —2—The evaluation of the model of posterior circulation ischemia in rabbits established by the method of deligation in thr

2、ee blood vessels Postgraduate: Yu Jianying Supervisor: Zhu Yi professor AbstractObjective: To evaluate the reliability of the model of posterior circulation ischemia in rabbits made by deligation in three blood vesse

3、ls. Methods: The 108 New Zealand rabbits were divided into 15 minutes (n=36), 30 minutes (n=36) and 120 minutes (n=36) after operation. The rabbits of above-mentioned time were matched according to sex, age, and weight,

4、then were randomly divided into the group of sham operation (n=12), the group of deligation in right common carotid artery (n=12) and the group of deligation in three blood vessels (right common carotid artery and double

5、 vertebral arteries) (n=12). The physiological indexes(heart rate, respiration rate, pupil diameter) of every group were examined and these rabbits were examined with PWI and DWI according to homologous time. After exam

6、ination two rabbits’ brains of every group were rapidly taken through decapitation and sliced according to the deck of MRI. The brain slices were stained in the liquor of two percent TTC. Another four rabbits’ hearts wer

7、e perfused with four percent paraformaldehyde and the ROI of two brains were taken to prepare for the specimens of pathology and the other for the specimens of electronmicroscope. Results: The physiological indexes of ev

8、ery group in homologous time had no significant difference (P>0.05). At 15 minutes after operation, the CBF decreased, the MTT extended in the rabbits of the group of deligation in three blood vessels and had signific

9、ant difference (P0.05). At the 30 minutes after operation, the CBF, the CBV decreased, the MTT extended in the rabbits of the group of deligation in three blood vessels versus other two groups respectively and had signif

10、icant difference (P0.05). At the 120 minutes after operation, the CBF, the CBV decreased, the MTT extended, the ADC decreased in the rabbits of the group of deligation in three blood vessels and had significant differenc