分子伴侶DJ-1與小肽協(xié)同抑制α-突觸核蛋白的聚集.pdf

1、湖北工業(yè)大學(xué)碩士學(xué)位論文分子伴侶DJ1與小肽協(xié)同抑制α突觸核蛋白的聚集姓名：徐銳申請學(xué)位級別：碩士專業(yè)：發(fā)酵工程指導(dǎo)教師：汪浩勇20090501湖北工業(yè)大學(xué)碩士學(xué)位論文AbstractParkinson’sdisease(PD)isacommonneurdegenerativedisease，whichhashighermorbidityandharmfultothehealthoftheelderlyneabnormalaggrega

2、tionofQsynuclein(aSyn)playscrucialimportantrolesinPDThemolecularchaperoneofDJ一1(PARKT)andsmallpeptidewhichsourcesfrom0‘Syn(∞VGGAVVTGV7q)caninhibittheaggregationofOL—SynWedesignedsixsmallpeptide(Pn：P1P6)whichfromthecorese

3、quenceofaSynWithsmallpeptidePc(MGGAVVTGR)usedasacontrol，theinhibitionontheaggregationofCtSyn(A53Tw工$129A)bythesmallpeptidesPn，byDJ一1plusPnwasstudiedusingvisualizationofproteinproteininteractioninbacterianemainconclusions

4、areasfollows：1Sixsmallpeptidescaninhibittheaggregationofa—Syn(A53TwT$129A)indifferentlevelsneinhibitionofaSyn(A53T)aggregationincreased51％bysmallpeptideP4(M(R)7KLMRVGGAVVR)comparedwithPCTheinhibitionofQSyn($129A)aggregat

5、ionincreased140％byP3(M(R)7KLMRVGGAVVTGR)comparedwithPcTheinhibitionof0【Syn(WT)aggregationincreased117％byP3(M(R)TKLMRVGGAVVTGR)comparedwithPe2DJ1canfunctionasmolecularchaperoneinvivoInteractionofDJ一1withsmallpeptideplayav

6、eryimportantroletoaffectstheinhibitionaggregationofasynucleininEcoliFoursmallpeptides(Pn：P1P4)werechosenfortheresearchTheCOexpressionofDJ1withsmallpeptideP1(MRGVVAAAR)canimprovetheinhibitionaggregationofaSyn(S129A)by29％；

7、T1leCOexpressionofDJ1withsmallpeptideP3(MRVGGAVVTGR)CallimprovetheinhibitionaggregationofaSyn(A53T)by134％；theCOexpressionofDJ1withsmallpeptideP4(MRVGGAVVR)canimprovetheinhibitionaggregationofaSyn(A53T)by129％，3Themethodof

8、twoplasmidcoexpressionWasfeasibleThesuccessfulapplicationoftwoplasmidCOexpressionsystemprovidesanewapproachfortheprotein—proteininteractions，nleapplicationofsmallpeptidewhichcaninhibittheaggregationofaSynwillbeabletoprev