胃癌-樹突狀細胞融合疫苗制備及其抗腫瘤生物學(xué)效應(yīng)研究.pdf

1、第三軍醫(yī)大學(xué)博士學(xué)位論文1英文縮寫一覽表英文縮寫英文全稱中文對照AgAntigen抗原AAminopterin氨基喋呤AIApoptosisIndex凋亡指數(shù)APCAntigenpresentingcell抗原提呈細胞bpBasepair堿基對cDNAcomplementaryDNA互補脫氧核糖核酸CPMCountsperminute每分鐘記數(shù)CTLCytotoxicTlymphocytes細胞毒性T淋巴細胞DCDendriticcel

2、l樹突狀細胞DEPCDiethylpyrocarbonate二乙酸焦磷酸酯DMSODimethylsulfoxide二甲基亞砜DNADeoxyribonucleicacid脫氧核糖核酸dNTPDeoxynnuclesidetriphosphate三磷酸脫氧核苷EBEthidiumbroe溴化乙錠FACSFlesceneactevatedcellster流式細胞記數(shù)FBSFetalbovineserum胎牛血清FCSFetalcalfse

3、rum小牛血清FCMFlowCytometer流式細胞儀FITCFluesceinisothiocyanate異硫氰酸熒光素HHypoxanthine次黃嘌呤3HTDR3Hlabeledthyine3H標記胸腺嘧啶脫氧核苷酸IFNInterferon干擾素IL10Interleukin10白細胞介素10KDKilodalton千道爾頓MHCMajhistocompatibilitycomplex主要組織相容性復(fù)合體MNCMononucl

4、earcell單個核細胞MOMonocyte單核細胞第三軍醫(yī)大學(xué)博士學(xué)位論文3StudyonthepreparationantitumbiologicaleffectsofgastriccancercelldendriticcellfusionvaccineAbstractObjective:Gastriccancerisoneofthemostcommonmalignanttumsinclinicalwk.Withthetraditi

5、onaltreatmentssuchasradicaloperationchemotherapyradiotherapypatientsstillhavetofaceonapoprognosis.Dendriticcellisthenewfocusofantitumbiotherapytheseyears.Manyresearchershavebeenattractedbyitsstrongabilitytopresentantigen

6、inspiremeeffectiveantitumimmunereactions.Gastriccancercellsarelackofdifferentialtumantigenswiththefmermethodsweonlycangetfewtumantigenstheirabilitytoirritatedendritccellssuccedentantitumimmunereactionsisverytiny.Inthisex

7、perimentweusedcellfusiontechnologytogetthegastriccancercelldendriticcellfusionvaccine.Wehopedthatthefusioncellscouldgetmetumantigeninfmationtoincreasetheirphenotypeshavemecompetencetoirritatetheantitumimmunereactions.Byt

8、hisexperimentwehopedtofindoutwhetherthefusionvaccinehassatisfyingsafetytoverifyitsabilityofinducingantitumimmunereactions.Byresearchingitsbiologicaleffectswehopedtogetanewkindofantitumvaccinefgastriccancer’sbiologicalthe

9、rapy.Methods:Part1.Peripheralbloodmononuclearcellswereseparatedfromgastriccancerpatientscoculturedwithgranulocytemacrophagecolonystimulatingfactsinterleukin4tumnecrosisfacttogeneratematuredendriticcells.Thefrozenstedhuma

10、ngastriccancerSGC7901celllineswereresuscitatedculturedatthesametime.ThedendriticcellsSGC7901cellswerefusionedbyusingofpolyethyleneglycolthepurefusioncellswerescreenedoutbyculturedwithHATHTiveculturesystems.Thephenotypeso

11、fdendriticcellsfusioncellsweredetectedbyflowcytometry.Part2.Thebiologicalacteristicsoffusioncells(suchastheirmphologicalspecialitiestheexpressionofcellsurfacephenotypes(CD80CD83CD1aHLADR)theirgrowthcurveswhethertheycanpr