消癥丸對膠質瘤C6細胞增殖抑制和誘導凋亡及Bcl-2表達影響的實驗研究.pdf

1、X777837學院代號專業(yè)代碼學號!!!!!!塑密級——陜西中醫(yī)學院ShaanxiUniversityofChineseMedicine碩士學位論文消癮丸對膠質瘤C6細胞增殖抑制和誘導凋亡及Bcl一2表達影響的實驗研究學位申請人李淑芳專業(yè)名稱中西醫(yī)結合臨床(腦病)申請學位類型臨床醫(yī)學碩士專業(yè)學位指導教師姓名協(xié)助指導教師陳美芳教授閏詠梅教授論文提交日期2005年4月nhibitionofproIiferationandinductiono

2、fapopotosisofC6gIiomaceJIsbyXiaozhengWanwithMedicatedSerumABSTRACTOjective：ToinvestigateXiaozhengWan(XZW)withMedicatedSerumontheproliferationandinducingtheapopotosisofC6gliomacellsMethods：UsingserumpharⅢacologicmethod，th

3、eC6cellswasobservedbyco—culturingwithdifferentdosagesofmedicatedserumcontainingXZWMTTwasusedtomeasurethelevelsoftheproliferationofC6culturedwithmedicatedserumcontainingXZWindifferentdosageandtimepointsTheinduceapopototic

4、ratewasobservedbyflowcytometry(FCM)ThemorphologicalchangeofC6cellswasdetectedbyelectronicmicroscopyImmunohistochemistrywasusedtoexaminetheexpressionofBcl一2aftertreatmentofmedicatedserumcontainingXZWResults：Thedifferentdo

5、sagesofmedicatedserumcontainingXZWcouldesignificantlyinhibittheproliferationofC6cells，andinhibitoryraterevealedamedicatedserumdose—timedependentmannerTheoutcomeofFCMshouldthattheapopototiccellratewasinadosedependentmanne

6、rThemorphologicalchangeofapopotosiswerealsofoundbyelectronicmicroscopyXZWdecreasedexpressionofBcl一2intheC6cellsConclusion：XZWinhibittheproliferationofC6，whichwasassociatedwithinhibitingtheexpressionofBcl一2，andinducetheap