GM6001在LASEK術后角膜損傷修復中作用的研究.pdf

1、石河子大學碩士學位論文GM6001在LASEK術后角膜損傷修復中作用的研究姓名：陳曉玲申請學位級別：碩士專業(yè)：眼科學指導教師：高曉唯20100501GM6001在LASEK術后角膜損傷修復中作用的研究GM6001在LASEK術后角膜損傷修復中作用的研究IIAbstractObjective：ToinvestigatetheeffectofGM6001oncnealdamagementrestationafterlaserepitheli

2、alkeratomileusismeanwhiletoexplethemechanismofcnealhealingafterlaserepithelialkeratomileusis.Methods：(1)TomakeuptheGM6001eyes(2)TobuildtheLASEKmodelsofrabbits(3)TwentysevenNewZealwhiterabbitsweredividedromlyintoGM6001gro

3、up、FMLgroupNCgroupCnealhazegradewereevaluatedbyslimlightat0.5、12mouthpostoperativeintervals.Tworabbitswereromlyharvestedineachgroupatdifferentpostoperativeintervalsthenexaminedbyopticaltransmissionelectronmicroscope(TEM)

4、、confocalmicroscopecollagentypeⅢexpressionbyimmunohistochemicalanalyses.Results：1.Slimlightofcneas:Cnealhazegradesat0.51monthaftersurgeryweresignificantdifferencesamongthegroups(P＜0.01)ComparingwiththeNCgroupTheCnealhaze

5、gradesinGM6001groupFMLgroupwereapparentlylower(P＜0.01)buttherewasnosignificantdifferencebetweenGM6001FMLgroups(P＞0.05).therewasnosignificantdifferencesamongthethreegroupsat2monthpostoperativelyaswell(P＞0.05).2.Pathologic

6、alsectionofcnealtissue:Thecnealepithelialcellsofexperimentalgroupswerehyperplasiaedthickevidentiyateachpostoperativeinterval.Thecnealepithelialcellswereincreasedto67layersComparingwiththeNCgroupthecnealstromacellswereles

7、sconcentratedattheablationzoneinGM6001groupFMLgroup.3.collagentypeⅢexpressionbyimmunohistochemicalanalyses:NegativeexpressionofcollagentypeⅢinnmalcnea.AtonemonthaftersurgerycollagentypeⅢwasstrongexpressedinNCgroup.Atonem

8、onthaftersurgerycollagentypeⅢwasweaklyexpressedinGM6001groupcollagentypeⅢwaspositivelyexpressedinFMLgroup.4.transmissionelectronmicroscope(TEM):ComparingwiththeNCgroupFMLgroupthenumbersoffibroblastatthefestromawerelessth

9、ecollagenfiberskeepedwellarrangedthecapacitiesoffibroblastsweresmallincnealstromainGM6001group.thecontentsofcellularganwerefewcellswerestableinGM6001group.5.Confocalmicroscopythroughfocusing(CMTF):ContrasttheGM6001groupw

10、iththeNCgroupFMLgroupthecnealstromacellswerealignedmeregular.ThenumbersofkeratocytesinGM6001groupwereapparentlylowerthaninNCgroupinFMLgroup(P0.01)，therewasdifferencebetweenNCgroupFMLgroup(P0.01).Conclusion：GM6001hasinhib

11、itiveeffectoncnealhazefmationafterLASEKwhichbysuppressedtheproliferationofkeratocytesdecreasedthesynthesisofcollagentypeⅢ.ThisefficacywassimilartoFML.ThemechanismofcnealhazefmationwasrelevanttoreleaseMMPsafteroperationth