酶膜耦聯(lián)反應(yīng)制備酪蛋白抗氧化肽的工藝研究

1、江南大學(xué)碩士學(xué)位論文酶膜耦聯(lián)反應(yīng)制備酪蛋白抗氧化肽的工藝研究姓名：高婷申請學(xué)位級別：碩士專業(yè)：食品科學(xué)指導(dǎo)教師：盧蓉蓉20091201AbstractAbstractEnzymaticproductionanduseoffunctionalandbioactiveproteinhydrolysatescontinuestogainprominenceinthefoodindustryConventionalproteinhydrolys

2、isperformedinthetraditionalbatchreactorisaverypopularprocess；Howeveritalsohasseveraldisadvantages，forexamplesitisverycostyandtheenzymeCallnotbereusedUseofultratitrationmembranescoupled、訪mahydrolysistanktoprovidesimultane

3、ousseparationofthehydrolysatesfromthereactantsisattractive，alternativeThemethodinvolveshydrolysisoftheproteinsinenzymaticmembranereactors(EMR)111ehydrolysisofcaseininanEMRwasstudiedinthispaperSevenenzymeswereusedtohydrol

4、yzecaseininthebatchreactoratfirst，andthenfourofthemwerechosentobeusedintheEMRThepropertiesofhydrolysateswerestudiedbyscavengingofDPPHfreeradical，fractionalconversion，molecularweightdistributionandaminoacidanalysisItwasfo

5、undthatAS1398Neutraseyieldedhydrolysates謝t11thebestpropertiesByresponsesurfaceregressionanalysis，theoptimumenzymehydrolysisconditionsofAS1，398Neutrasewereachievedassubstrate([S])30mg／mL，time90min，temperature(T)50“C，enzym

6、e／substrateratio([E]／[S])2％(w／w)，andpH72forthebatchprocessFurthermore，caseinhydrolysatespreparedunderthisconditiongaveastrongantioxidantactivity，withaDPPHradicalscavengingavtivityof8043O47％(10mg／mL)Basedonmolecularweight

7、distributionofpermeateandtheintegratedeffectofthewholeprocess，3kDa，5kDaand10kDapoly(ethersulfone)membraneswereusedintheEMRprocessIntheoptimumhydrolysisconditionsat30kPaobtainedfrom10kDamembraneshowedthehighestantioxidant

8、activitycomparedwimthe3kDaand5kDamembranesIt’S1(750ofDPPHOandOHwere537、515and121mg／mLrespectivelyItalsogavethebestfluxCleanmembraneresistancewas034minkPa／mLandpolarisationlayerresistancewas123minkPa／mL111eeffectofprocess

9、ingconditionsinEMILonthepermeates’propertieswerestudiedTheresultsshowedthatattheoptimalconditionof25。30kPa【s]initial30mg／mL，[E】initial2一25％，thepermeateshadstrongantioxidantactivity謝t11permeatefluxof1163mL／min，afreeradica

10、l—scavengingrateof671％andfractionalonversionof5991％ThehydrolysatespreparedfromtraditionalbatchreactorwerecomparedintheabilitytoscavengedifferentfreeradicalsTheresultsshowedthatICsoDPpHwere725mg／mLand489mg／mL。ICsoonwere69