轉基因冰草后代植株的分子檢測與抗性研究.pdf

1、內(nèi)蒙古農(nóng)業(yè)大學碩士學位論文轉基因冰草后代植株的分子檢測與抗性研究姓名：董淑君申請學位級別：碩士專業(yè)：草畜種質資源學指導教師：米福貴王桂花20090501IdentificationandResistanceDetectionofFutureGenerat。TransgenicWhea!=tgeneratlon1railsen11eaIgrass^bstractSelfCROSSfuturegeneration(Tlgeneration)

2、ofp5CStransgenicwheatgrasswereusedasmaterialinthisstudyBymolecularandresistencedetermination，stablytransferredwheatgrasswereobtainedandtheintegrationandexpressionofexogenousgenepSCSinToandTIgenerationtransgcnicwheatgrass

3、wereconfirmed，whichprovidetheoreticalbasisforthelaterstudyoftransgenicwheatgrasses；atthesametime，transgenicwheatgrasswithstrongresistancewereidentifiedbydetectingtheredroughtandsalinetolerance，aSbasicmaterialsforbreeding

4、hightolerancevarietiesnextstepThepreliminaryfmdingsofthisstudyareasfollows：ITheexogenousgenep5CShaveintegratedintothegcnomicDNAofTIgenerationtransgenicwheatgrassandtheintegrationnumberis1～2，whichconfmnedbyPCRandSouthernb

5、lotanalysisNorthernblotanalysisshowedthatsomeoftheexogenousgeneintheTlgenerationmaIlsgemcwheatgrassexpressedattranscriptionallevelThereare58plantspresentedpositiveresultinatotalof128TIgenerationtransgenicwheatgrassinPCRa

6、nalysis2Underdroughtstressandsaltstress，thechangeofchlorophyllcontentandsolublesugarcontentofToandTIgenerationtransgenicwheatgrassshowednodifferencecomparedwithnontransgenicwheatgrassOnthe14tllclayofintimidation，theenzym

7、eactiveofSOD、POD、CATintransgeniewheatgrasswerealittlehigherthannontranSgenlcwheatgrassTheLeafrelativewatercontentandProcontentoftransgenicwheatgrasswerehigherthannontransgenicwheatgrass；ThemembranepermeabilityandMDAconte

8、ntoftransgenicwheatgrassincreasedlessthannontmmgcnicwheatgrassTakingalltheseresults，thetoleranceoftransgenicwheatgrasswerebetterthannontransgenicwheatgrass3TheProcontentofToandTtgenerationtransgenicwheatgrassCanIncreased