雷帕霉素納米粒局部處理兔離體靜脈對防治其移植后狹窄的作用

1、雷帕霉素納米粒局部處理兔離體靜脈對防治其移植后狹窄的作用鞏性軍曹廣慶張希全吳樹明張供李躍華周敏[摘要]目的研究用納米緩釋技術處理的雷帕霉素(RPMNP)對移植靜脈狹窄的防治作用。方法選取家兔40只，建立兔頸外靜脈間位移植至頸總動脈的移植靜脈模型，按處理方式不同，分為4組：藥物納米局部干預組（RPMNP局部處理頸外靜脈，n=10，A組）、藥物納米全身干預組(RPMNP周圍靜脈滴注，n=10，B組)、空白納米局部干預組（空白納米粒局部處理頸

2、外靜脈，n=10，C組）和未干預組（不做上述處理，n=10，D組）。術后28d，切取各組移植靜脈和對側正常靜脈，病理學檢測移植靜脈內膜厚度、內徑、內膜中膜厚度比以及膠原容積指數。結果移植靜脈的組織病理學檢測結果表明，A、B、C、D組移植靜脈和對側正常靜脈的內膜中膜厚度比分別為0.260.02、0.730.05、0.710.04、0.690.03、0.240.01，膠原容積指數比分別為0.240.03、0.560.06、0.530.07、

3、0.490.08、0.210.01。B、C、D組的組織病理學（移植靜脈內膜厚度、內徑、內膜中膜厚度比以及膠原容積指數）與對側正常靜脈組比較，均出現明顯的病理學改變，差異均有統(tǒng)計學意義(均P＜0.05)；3組之間移植靜脈的組織病理學結果比較，差異均無統(tǒng)計學意義（均P＞0.05）。而A組移植靜脈的組織病理學結果與對側正常靜脈比較，差異無統(tǒng)計學意義(P＞0.05)；與其他3組比較，差異有統(tǒng)計學意義(P＜0.05)。結論采用納米緩釋技術處理的雷

4、帕霉素，對離體的移植靜脈行管腔內局部處理能抑制移植靜脈內膜增生防治移植靜脈狹窄。西羅莫司；納米粒子；局部治療；移植靜脈；狹窄PretreatmentofisolatedveinwithrapamycinnanopartidesinhibitsveingraftstenosisinrabbitsGONGXingjunCAOGuangqingZHANGXiquanWUShumingZHANGGongLIYuehuaZHOUDepartmen

5、tofCardiovascularSurgeryQiluHospitalofShandongUniversityJinan250012China[Abstract]ObjectiveToexploretheeffectsofpretreatmentofcarbopolencapsulatedrapamycinloadednanoparticles(RPMNP)onveingraftstenosisinarabbitveingraftmo

6、del.MethodsAsegmentofcommoncarotidarterywasreplacedwithasegmentofexternaljugularveinin40rabbits.Theywereseparatedintofourtreatmentgroupsi.e.GroupA:veingraftswerepretreatedwithintraluminalRPMNPperfusionGroupB:peripheralve

7、nousveinswereinjectedwithRPMNPGroupC:veingraftsreceivedanequivalentperfusionofemptyvehicleGroupD:veingraftsreceivednotreatment.AtDay28postoperationthegraftsandnormalveinswereharvestedforhistologicalexaminationstoanalyzet

8、heindicatorsofintimalthicknessinternaldiameterintimalmediathicknessratioandcollagenvolumeindex.ResultsAtDay28postoperationtheintimalmediathicknessratioswere0.260.020.730.050.710.040.690.03and0.240.01inGroupsABCandDandthe

9、normalveinthecollagenvolumeindex0.240.030.560.060.530.070.490.08and0.210.01respectively.Comparedwiththenormalveinsthepathologicalindicatorsofveingraftintimalthicknessinternaldiameterintimalmediathicknessratioandcollagenv

10、olumeindexhadsignificantdifferencesinGroupsBCandD(allP＜0.05).Buttherewerenosignificantdifferencesamong3groups(allP＞0.05).Comparedwiththenormalveintheparametersofveingraftintimalthicknessinternaldiameterintimalmediathickn

11、essratioandcollagenvolumeindexhadnosignificantdifferenceinGroupA(allP＞0.05).ButascomparedwithothergroupstheseindicatorshadstatisticalsignificantdifferenceinGroupA(allP＜0.05).ConclusionThelocalpretreatmentofisolatedveinwi

12、thrapamycinnanoparticlesmayinhibitneointimalhyperplasiaandpreventeffectivelyveingraftstenosis.SirolimusNanoparticlesLocaltreatmentVeingraftStenosis10.3760cma.j.issn.03762491.2011.46.017山東省衛(wèi)生廳醫(yī)藥衛(wèi)生發(fā)展科技項目(2007HZ057)250012濟南