雷帕霉素納米粒局部處理兔離體靜脈對(duì)防治其移植后狹窄的作用

1、雷帕霉素納米粒局部處理兔離體靜脈對(duì)防治其移植后狹窄的作用鞏性軍曹廣慶張希全吳樹(shù)明張供李躍華周敏[摘要]目的研究用納米緩釋技術(shù)處理的雷帕霉素(RPMNP)對(duì)移植靜脈狹窄的防治作用。方法選取家兔40只，建立兔頸外靜脈間位移植至頸總動(dòng)脈的移植靜脈模型，按處理方式不同，分為4組：藥物納米局部干預(yù)組（RPMNP局部處理頸外靜脈，n=10，A組）、藥物納米全身干預(yù)組(RPMNP周圍靜脈滴注，n=10，B組)、空白納米局部干預(yù)組（空白納米粒局部處理頸

2、外靜脈，n=10，C組）和未干預(yù)組（不做上述處理，n=10，D組）。術(shù)后28d，切取各組移植靜脈和對(duì)側(cè)正常靜脈，病理學(xué)檢測(cè)移植靜脈內(nèi)膜厚度、內(nèi)徑、內(nèi)膜中膜厚度比以及膠原容積指數(shù)。結(jié)果移植靜脈的組織病理學(xué)檢測(cè)結(jié)果表明，A、B、C、D組移植靜脈和對(duì)側(cè)正常靜脈的內(nèi)膜中膜厚度比分別為0.260.02、0.730.05、0.710.04、0.690.03、0.240.01，膠原容積指數(shù)比分別為0.240.03、0.560.06、0.530.07、

3、0.490.08、0.210.01。B、C、D組的組織病理學(xué)（移植靜脈內(nèi)膜厚度、內(nèi)徑、內(nèi)膜中膜厚度比以及膠原容積指數(shù)）與對(duì)側(cè)正常靜脈組比較，均出現(xiàn)明顯的病理學(xué)改變，差異均有統(tǒng)計(jì)學(xué)意義(均P＜0.05)；3組之間移植靜脈的組織病理學(xué)結(jié)果比較，差異均無(wú)統(tǒng)計(jì)學(xué)意義（均P＞0.05）。而A組移植靜脈的組織病理學(xué)結(jié)果與對(duì)側(cè)正常靜脈比較，差異無(wú)統(tǒng)計(jì)學(xué)意義(P＞0.05)；與其他3組比較，差異有統(tǒng)計(jì)學(xué)意義(P＜0.05)。結(jié)論采用納米緩釋技術(shù)處理的雷

4、帕霉素，對(duì)離體的移植靜脈行管腔內(nèi)局部處理能抑制移植靜脈內(nèi)膜增生防治移植靜脈狹窄。西羅莫司；納米粒子；局部治療；移植靜脈；狹窄PretreatmentofisolatedveinwithrapamycinnanopartidesinhibitsveingraftstenosisinrabbitsGONGXingjunCAOGuangqingZHANGXiquanWUShumingZHANGGongLIYuehuaZHOUDepartmen

5、tofCardiovascularSurgeryQiluHospitalofShandongUniversityJinan250012China[Abstract]ObjectiveToexploretheeffectsofpretreatmentofcarbopolencapsulatedrapamycinloadednanoparticles(RPMNP)onveingraftstenosisinarabbitveingraftmo

6、del.MethodsAsegmentofcommoncarotidarterywasreplacedwithasegmentofexternaljugularveinin40rabbits.Theywereseparatedintofourtreatmentgroupsi.e.GroupA:veingraftswerepretreatedwithintraluminalRPMNPperfusionGroupB:peripheralve

7、nousveinswereinjectedwithRPMNPGroupC:veingraftsreceivedanequivalentperfusionofemptyvehicleGroupD:veingraftsreceivednotreatment.AtDay28postoperationthegraftsandnormalveinswereharvestedforhistologicalexaminationstoanalyzet

8、heindicatorsofintimalthicknessinternaldiameterintimalmediathicknessratioandcollagenvolumeindex.ResultsAtDay28postoperationtheintimalmediathicknessratioswere0.260.020.730.050.710.040.690.03and0.240.01inGroupsABCandDandthe

9、normalveinthecollagenvolumeindex0.240.030.560.060.530.070.490.08and0.210.01respectively.Comparedwiththenormalveinsthepathologicalindicatorsofveingraftintimalthicknessinternaldiameterintimalmediathicknessratioandcollagenv

10、olumeindexhadsignificantdifferencesinGroupsBCandD(allP＜0.05).Buttherewerenosignificantdifferencesamong3groups(allP＞0.05).Comparedwiththenormalveintheparametersofveingraftintimalthicknessinternaldiameterintimalmediathickn

11、essratioandcollagenvolumeindexhadnosignificantdifferenceinGroupA(allP＞0.05).ButascomparedwithothergroupstheseindicatorshadstatisticalsignificantdifferenceinGroupA(allP＜0.05).ConclusionThelocalpretreatmentofisolatedveinwi

12、thrapamycinnanoparticlesmayinhibitneointimalhyperplasiaandpreventeffectivelyveingraftstenosis.SirolimusNanoparticlesLocaltreatmentVeingraftStenosis10.3760cma.j.issn.03762491.2011.46.017山東省衛(wèi)生廳醫(yī)藥衛(wèi)生發(fā)展科技項(xiàng)目(2007HZ057)250012濟(jì)南