CXCL12-CXCR4軸在臍血間充質(zhì)干細(xì)胞影響白血病細(xì)胞增殖和凋亡中作用的研究.pdf

1、分類號(hào)：R733密級(jí)：不保密UDC：610學(xué)校代碼：11065碩士學(xué)位論文CXCL12CXCR4CXCL12CXCR4軸在臍血間充質(zhì)干細(xì)胞影響白血病細(xì)胞增殖和軸在臍血間充質(zhì)干細(xì)胞影響白血病細(xì)胞增殖和凋亡中作用的研究凋亡中作用的研究李明穎指導(dǎo)教師趙春亭教授學(xué)科專業(yè)名稱內(nèi)科學(xué)（血液病學(xué)）論文答辯日期2017年5月26日ⅡTheeffectofCXCL12CXCR4signalaxisontheproliferationapoptosisof

2、leukemiccellsculturedwithhumanumbilicalcdbloodderivedmesenchymalstemcellsAbstractObjective:ToinvestigatetheroleofCXCL12CXCR4signalaxistheeffectsofGCSFAMD3100ontheleukemiccellstheirmicroenvironmentinthecoculturemodelofHUC

3、MSCswithHL60(acutemyeloidleukemiacellline)cellsJurkat(acutelymphoblasticleukemiacellline)cells.Methods:HL60cellsJurkatcellswerecoculturedwithhumanumbilicalcdbloodmesenchymalstemcells(HUCMSCs)themodelwasmanagedwithGCSFAMD

4、3100theircombinationrespectively.CellviabilitycellcycleweremeasuredbyCellCountingKit8(CCK8).CellapoptosiswasassessedbyflowcytometryusingtheAnnexinVApoptosisKitaswellasthecellcycleanalysis.TheexpressionofsurfaceCXCR4prote

5、intotalCXCR4proteinofleukemiccellsweredetectedbyflowcytometryWesternblotrespectively.Results:HUCMSCscoulddecreasetheviabilityofHL60cellsJurkatcellsthepercentageofapoptoticcellstheycanalsoincreasethenumberofquiescentcells

6、whileGCSFAMD3100couldfurtherreducetheproliferationofHL60cellsJurkatcellsinHUCMSCcoculturemodeldestructedtheantiapoptoticeffectofHUCMSConHL60cellsJurkatcellsthecombinationoftwodrugsresultedinasynergisticeffect.GCSFcouldre

7、ducetheexpressionofsurfaceCXCR4proteintotalCXCR4proteininleukemiccellswhileAMD3100couldonlydecreasetheexpressionofCXCR4onthesurfaceofleukemiamembranehavingnoeffectontheexpressionofCXCR4proteinincytoplasm.Conclusion:Human

8、umbilicalcdbloodmesenchymalstemcellscaninhibittheproliferationapoptosisofacuteleukemiacellsincreasethenumberofG0G1phasecellsinleukemiccells.AMD3100candecreasetheexpressionofsurfaceCXCR4proteininleukemiacellsGCSFcandecrea

9、setotalCXCR4proteinaswellasmembraneCXCR4proteinexpression.BothofthemcanblocktheCXCL12CXCR4signalaxisweakeningtherelationshipbetweenleukemiacellsmicroenvironment.onthebasicofHUCMSCinfluencedleukemiacellsgrowthproliferatio