固定化細(xì)胞技術(shù)制備L-苯丙氨酸.pdf

1、江南大學(xué)碩士學(xué)位論文固定化細(xì)胞技術(shù)制備L苯丙氨酸姓名：冷泳申請學(xué)位級別：碩士專業(yè)：發(fā)酵工程指導(dǎo)教師：孫志浩20060601江南大學(xué)碩士學(xué)位論文AbstractE，c加，fc?；譪o，fSw0209—52，arecombinalltstrainwithhi曲transanlinaSeactivity，wasimmobilizedandwasusedtoprepareL—phenylalanineinpackedbedreactoLL—ph

2、enylalallineextraction行omthebiotransf0肌ationsolutionwasalsostudiedjntheresearchThecellsofE∞，fSW0209—52wereentrappedbyKcarrageenana11dweretreatedby91utamldehydeaRerimmob訂izationTheactivityyieldoftheimmobilizedcellswas73％V

3、ariouscharacteristicsofirnmobmzedcellswereassessedTheoptimalpHforconversionwas8O，t11eoptimaltemperatl】rewas37oCa11dtheoptimalamountof5一PLPwaS100“m01／LThestomgestabilitya11dt11eoperationalstabilit)r、veregreatlymlpmvedaRer

4、immobilizationTheeffectof5一PLPandVB6onconVersionbyimmobilizedcellswascomparedAsVB6canbeconvertedto5一PLPwith√氣：IEitcansubstitute5PLPatacertainextent，butthestabilityofimmobilizedcellswasdecreasedComp撕ngt11ee虢ctofI／Donconti

5、Iluousoperation，tllepackedbedreactor謝tll133H／DratiowaschosenforcontinuousproductionE行bctsofsubstrateconcentmtiona11dresidencetimewereinVestigatedConsideringbothconverSionyieldandvolumetricproductivi吼stlldiesontheoperatio

6、nalstabilityofthereactorwereconductedundertheconditionof309／LPPAfeedingconcentrationand10hresidencetimtThesystemcouldbeoperatedfor40dwitlloutsigIlificamdeclineaIldtlleavemgeproductivitywaS185∥mandtheconversionyieldwasabo

7、Ve85％tllrou曲outt11e40daysTheextractionofL巾henylalalline舶mbiotransfbmationsolutionbyionexchaIlgeresinJK006wasalsostudiedE毹ctsoffactorsaboutadsorption，elutionanddecolorationwcreinVestigated1飛eoptimal叩erationconditions、vere

8、aSfollowing：bioⅡa11sfo砷ationsolutionpH2O，concentration5∥L，adsorbiIlgtemperature20oc，eluallt1mol幾NH3AfterdecoIorationbyresinD4006，theemuentwasconcen訂ated柚dcryStallizednlepurityofL—phenylalaIlinecrystal、vas967％andt11eyield

9、oftlletotalpmcesswas828％Therawmaterialcostoft11eentireprocesswaSes岫atedarmt11ebio脅sfbml缸ioncostofproductionwitll5一PLPa11dVB6respectiVelywascomparedAltlloughthecostofimmobilizationwasincreased，t11ewholecostofbiotransfomat

10、ionwitllVB6ascoenzymewasdecreasedInordertobringtlleprocessofproductionofLphenylalaIlinewimimmobilizedcellsindustrializatiOn，5一PLPimmobilizationaIldmethodsofimpmvingthestabilityofimmobilizedcellsintheprocessofproductionwi