MiRNA機制相關蛋白在不同類型慢性鼻-鼻竇炎中的表達.pdf

1、華中科技大學碩士學位論文MiRNA機制相關蛋白在不同類型慢性鼻鼻竇炎中的表達姓名：張雅娜申請學位級別：碩士專業(yè)：耳鼻咽喉頭頸外科學指導教師：劉爭201104華中科技大學碩士學位論文華中科技大學碩士學位論文3ABSTRACTObjective:ToinvestigatethedifferentexpressionofmiRNAmachineryproteinsinthehumannoseofhealthyindividualspatien

2、tswithchronicrhinosinusitiswithoutnasalpolyps(CRSsNP)，eosinophilicchronicrhinosinusitiswithnasalpolyps(EosCRSwNP)noneosinophilicchronicrhinosinusitiswithoutnasalpolyps(NonEosCRSwNP).toelucidatethesignificanceofmiRNAmachi

3、neryproteinsinthedevelopmentofchronicrhinosinusitis.Method:TheexpressionofmiRNAmachinerycomponents(Dsha、Dicer、PACT、TRBP、EIF2C2、FXR1)inhumannasalbiopsieswasdeterminedwithrealtimeRTPCR.Thenimmunohistochemistryserialsection

4、swereusedtofuthervertifythepresenceofmiRNAmachineryproteinsinhumansinonasalmucosa.Results:TheexpressionofDsha、Dicer、PACT、TRBP、EIF2C2、FXR1wasfoundinalmostallspecimenstestedbymeansofrealtimeRTPCRbutexceptfPACTnodifferences

5、wereseenamongthefourgroups.ThemRNAlevelofPACTwassignificantlyhigherinpatientswithEosCRSwNPNonEosCRSwNPwhencomparedwithhealthyindividualsanelevatedPACTlevelwasseeninEosCRSwNPwhencomparedwithCRSsNP.Immunohistochemicalanaly

6、sisrevealedexpressionofPACTinbiopsiesofEosCRSwNPNonEosCRSwNPwasobviouslyhigherthanthatofnmalsinonasalmucosaCRSsNPrespectively.ImmunohistochemicalserialsectionsshowpresenceofPACTinCD138plasmacells.MeanwhilethetendencyofCD

7、138plasmacellsinbiopsiesamongthefourgourpsisconsistentwiththetrendofPACTlevelofPACTispositivelyrelatedwithCD138whichhasastrongsignificanceinourexperiment（r=0.741P=0.000）.Conclusion:TheoverexpressionofmiRNAmachineryprotei

8、nPACTinEosCRSwNPNonEosCRSwNPmayimplyanabnmalexpressionofmiRNAsinCRS.PACTmainlyexpressedincytoplasmaofplasmacellswhichsuggeststhatregulatymechanismofmiRNAisinvolvedinregulationofplasma’sfunctionleadstonasalpolyps.Keywds:M