鼠源多形擬桿菌的分離及其對小鼠生長性能和脂類代謝的作用.pdf

1、1108015分類號密級單位代碼：10019學號：S05030471絮目差≮文等碩士學位論文鼠源多形擬桿菌的分離及其對小鼠生長性能和脂類代謝的作用BacteroidesthetaiotaomicronIsolatesfromtheconventionalMiceandItsEffectsonGrowthPerformanceandLipidMetabolisminMice研究指導教生：直塑師：張日俊教授申請學位門類級別：盔鱟亟向：：塑盤

2、生塹掛盔二Oo七年五月名方學業(yè)究在專研所中國農(nóng)業(yè)大學碩士學位論文第一章目錄AbstractInthisstudyWeadoptedimprovedinediillmfortheselection磺Bthetaiotaomicronandconductedtoinvestigateeffects斌BthetaiotaomicronOngrowthperformanceandlipidmetabolisminmiceExperimentl：

3、InmisstudyWeedup把dimprovedmediumfortheselectionoftheBfra#腦groupfromtimcoloncontentsofconventionalmiceThroughmorphologyphysiologicalandtermentationcharacteristics日：∞ebactedarepremtedspecies研‘BthetaiotaomicronandBvu／gatusW

4、estoredthetwoswainsforalongtimebythemethodofv毫翰珊丘氍耀甜ryin呂andwestudiedeffectsof置thetaiotaomicronongrowthperformanceandlipidmetabolisminmiceExperiment2：Eighty20ageKonmingmicewmrandomlyallocatedintofourgroups(A，B。C，D)with4r

5、eplicatesandeachgroupcontained20miceAWaScontrolgroupBwaslowlevelbaOe—ummodelgroupinwhicheachmousewasiII扛鹋a矧cinfusionampicillin05gcontinuouslyforthreedaysinordertoestablishlowlevelbacteTinnlmicemodelIngroupC20micewereadmi

6、nisteredwithImlAthetaiotaomicronliquidfermentationbygastricinfusiontwiceadayIngroupDfirstweestabliashedthelowlevelb鋤刪岫modelandthenthe20micamodelwereintragastricedthes鋤eba曲涮liquidfermentintlThepurposeofthisexperimentWusto

7、investigatetheBacteriode8伽successioninfourdifferentphysiologicalconditionTheresultshowedthatthegroupBCWIestablishalowlevelbacillusenvironmentanditCanI【e印thissituationfor16daysComparedtothegroupAandCtheanaountofBacteriode

8、singroupBWasdecreasedsignificantly(P005)ThegroupCc∞makethemcc％sioIlalpe酞aheadoftheCommontimeanditCanalsoimprovethepeaknothalf(4％)SuccessionaltrendlineinGroupDcousistentedwithgroupC，butthepeakwaslowerthanthegroupCExperime

9、nt3：Thedesignationoftheexperiment3Wasthesamewiththeexperiment2ThisstudyWaSconductedtoinvestigatethefeasibilityoflowlevelbacteriummodelandstudiedtheeffectsof曼thetaiotaomicronOilgrowthperformanceandlipidmetabolisminmiceThe

10、resultobtainedshowedthatthegroupBhadestablishedtheenvironmentoflowlevelbacteriumwhichcausedgreatsideeffectonthemodelmiceinB，DComp口edt0groupA，thebodyweightofthemiceingroupDwasdecreasedsignificantlyfP《o05)Duringtheexperime

11、ntthebodyweightanddailygainofgroupDwerehigherthangroupB，andintheandoftheexperiment(from28thto34thday)hadarapidlyincreasephaseThephenomenonshowedthat置thetaiotaomicroncouldrevivethesideeffectofantibioticsThebodyweightandda

12、ilygainingroupCwerehigherthanothertreatmentsandthebodyweightWasincreasedsignificantlyfPo05)from19thto22thdayBiochemicalanalysisWasmadeafterbloodsamplewerecollectedandliverandfattissurewerepreservadTheresultrevealedthat丑t

13、hetaiotaomicrontrendedtoacceleratethelipidmetabolisminmiceInconclusion置thetaiotaomicronhadtrendancetoincreasethegrowthperformanceacceleratetheabil姆oflipidmetabolism，andrevivethesideeffectofantibioticsKeywords：置thetaiotao