維生素K3增強吉西他濱誘導人膀胱癌T24細胞凋亡的研究.pdf

1、山西醫(yī)科大學碩士學位論文維生素K3增強吉西他濱誘導人膀胱癌T24細胞凋亡的研究姓名：趙亮申請學位級別：碩士專業(yè)：泌尿外科學指導教師：劉俊生20090320lJJCti醫(yī)科人學碩Jj學位論文EffectofvitaminK3ongemcitabineinducedapoptosisofbladdercancercelllineT24AbstractBackground：Gemcitabine(Gem)wasanewanti—pyrimid

2、inenucleotidemeta—bolismofchemotherapydrug，whichhadabroadanti—tumorspectrum，auniquemechanism，andlowtoxicitywithoutcross—resistanceandnonoverlappingtoxicitycharacteristicswhenusedwithotherchemotherapydrugsAlotofresearchin

3、cludinginvitrostudies，animalexperimentsandclinicaltrialsallindicatedthatGemhadagoodanti—tumoractivitytoavarietyofsolidtumors，whichhadbeensubstantialappliedtochemotherapyforadvancedbladdercancerinrecentyearsTheclinicalval

4、ueofGemhadbeenfullyaffirmedandthatwouldbeahotissueonthecombinationchemotherapyofGembasedonclinicalresearchResearcheshadshowedthatvitaminK3couldenhancetheanti—tumoractivitiesofavarietyofchemotherapydrugswhencombinedCurren

5、tllyithadbecameahotissueonthecombinationofvitaminK3andcertainfirstlinechemotherapydrugs，howevertheresearchofcombinationwithGemisstillrareInourpreliminarysmdDweobservedthecellapoptosisofvitaminK3combinedwithGemactingonhum

6、anbladderCanCereelllineT24Objective：ToinvestigatetheeffectofvitaminK3(VK3)combinedwithGemonthecellapoptosisandcellcycleofhumanbladdercancercelllineT24Method：(1)MTTwasusedtoselecttheminimumeffectiveconcentrationofGemwithc

7、ertainconcentrationgradient(001，01，10，100gg／m1)forinhibitingT24cellgrowthafter72hculture(2)ThenMTTWasusedtoselecttheminimumeffectiveconcentrationofcombinationofGemandVK3withcertainconcentrationgradient(2，5，10，209mol／L)fo

8、rinhibitingT24cellgrowthafter72hculture(3)Therewouldbethreegroupsandcultivateeachgroup：controlgroup，theminimumeffectiveconcentrationofGemgroupandtheminimumeffectiveconcentrationofcombinationgroup(4)Theapoptosiswasdetecte

9、dbytheflowcytometrywithPI，andthecellcyclewasanalyzedbyFACSstreamingsoftware(5)MicroscopeWasusedtoobservethechangesofthecellmorphologyineachgroupResults：(1)TheMTTselectedminimumeffectiveconcentrationofGemforinhibitingT24c

10、ellgrowthafter72hculturewas1099／m1(2)TheMTTselectedminimumeffectiveconcentrationofGemandVK3forinhibitingT24cellgrowthafter72hculturewas1099／mlGemand101xmol／LVK3group(3)TheresultofflowcytometryindicatedthattheapoptosisIII