木黃酮、三氧化二砷單獨及聯(lián)合應(yīng)用對慢性粒細胞白血病細胞增殖的實驗與臨床研究.pdf

1、蘇州大學碩士學位論文木黃酮、三氧化二砷單獨及聯(lián)合應(yīng)用對慢性粒細胞白血病細胞增殖的實驗與臨床研究姓名：支雅軍申請學位級別：碩士專業(yè)：內(nèi)科學指導教師：張日2000.11.1●一I，魚型：壘!壘苧塑墨壁魚窒旦翌!!些塑墮塑墮盟塞壁量墮墮竺壅墨壅塑墨Abstractobjective：InordertofindthemoreeffectivewaytotreatCML，weexploretheeffectofGenistein(GEN)、As2

2、03aloneorincombinationontheproliferationofCMLcells，andtoevaluatethetolerance，efficacyandtoxic耐ofAs201forthetreatmentofpatientswithCMLinAPMethods：Theeffectwasobservedbycellproliferation，cellviability，cellmorphologycolonyf

3、ormation，agarosegelelectrophoresis，celldifferentiation，RTPCRandFCMassays，andsevenadultpatientsdiagnosedasCMLinAPwerereceived10～15mg／dofAs203bycontinousinfusionaccordingtothecountsofperipheralwhitebloodcellsResults：As203o

4、f25umol／LorGENof5mg／Lfor2dayscouldinhibitthecellproliferationandinducetheapoptoticmorphologyandDNAfragmentationofCMLcells，andGENcouldinducetheCMLcellstoundergoerythroiddifferentiationTheycanalSeducethelevelofbclXLmRNABot

5、hofthemshowedsynergicphenomenonTheyallcouldmakeK562cellsinG2phaseincreasewhilethecellsinG1phaseandSphasedecreasesignificantlyThesee髓ctswerealltimedependentanddose—dependent3of7patientsachievedCRandtheothersPRfollowingmed

6、iantreatmentdurationof405daysAllpatientswerewelltolerabletoarsenicandshowedtheclinical，hematologicalandCFU—GMresponses，butnotimprovementofPhchromosomeandbcr／ablfusiongeneConelusion：(1)GENandAs，O，caninhibitthegrowthofK562

7、cellsandfreshCMLcellsrespectivelyorsynergisticallyThemechanismofgrowthinhibitionmightbeassociatedwithinducingapoptosisanderythroiddifferentiation。(2)As203couldhavethetherapeuticbenefittoCMLinAPbyinducingtheapoptosisofCML