EGCG聯(lián)合5-FU抑制結(jié)腸癌HT-29細(xì)胞生長及其機制的實驗研究.pdf

1、蘇州大學(xué)碩士學(xué)位論文EGCG聯(lián)合5FU抑制結(jié)腸癌HT29細(xì)胞生長及其機制的實驗研究姓名：周萍申請學(xué)位級別：碩士專業(yè)：消化內(nèi)科指導(dǎo)教師：陳衛(wèi)昌20080501EGCG聯(lián)合5FU抑制結(jié)腸癌HT29細(xì)胞生長及其機制的實驗研究英文摘要TheStudiestoAntiTumorEffectanditsMechanismsof()epigallocatechin3gallatet，Eccc)CombinedWith5FUonHumanColonCa

2、ncerCellLineHT29Invi仃oAbstractObjective：Tostudytheanti—tunloreffectanditsmechanismsof(一)epigallocatechin3gallate(EGCG)combined、析th5FUonhumancoloncancercelllineHT29invitroMethods：Invitro，humancoloncancgrcelllineH，r29wastr

3、eatedbyEGCGand㈣5FUinvariousconcentrationsrespectivelyaloneorincombinationTheinhibitoryeffectofcellgrowthwasassayedbyMTrmethod；cellcyclesandapoptosiswereanalyzedbyfloweytometry(FCM)；theexpressionsofERK、Akt、bcl2、baxwereana

4、lyzedbyWesternBlotResults：TheinhibitoryeffectofEGCGor5FUOlltheproliferationofHT29cellwerefoundindoseandtimedependentmanner(P005)ComparingwithEGCGor5FUalonetheinhibitoryeffectofEGCGcombinedwith5FUWassignificantlyincreased

5、(O05)ComparingwithcontrolgroupthecellapoptosisWasinducedbyEGCGor5FUaloneat48hours，thecellapoptosisratewassignificantlyincreasedbYEGCGcombinedwith5FU(P005)MeanwhiletheratioofG0／G1phasewasincreasedandtheratioofSphasew嬲decr

6、easedComparingwithEGCGor5FUalone，G0／G1phasewasincreasedandSphaseWasdecreasedsignificantlybyECCGcombinedwith5FU妒o05)TheexpressionofpERK、pAktandbcl2weredownregulatedandbaxwasupregulatedbyEGCGor5FUalonetheexpressionofpERK、p

7、Aktandbcl2weredownregulatedandbaxwasupregulatedsignificantlybyEGCGcombinedwith5FU(尸005)Conclusion：ThegrowthofliT一29cellinvitrowasinhibitedbyEGCGor5FUandindoseandtimedependentmaimerTheanticarcinomaeffectWassignificantlyen

8、hancedbyEGCGcombined塒tll5FU，andmaybeitsmechanismswerearrestingcellcycle，inducingcellapoptosisthroughdownreglatingtheexpressionsofp—ERK、pAktandbcl2andupregulatingtheespressionofbaxKe)rwords：Coloncancer；EGCG；5FU；p—ERK；p—Ak