苦參素對(duì)L02、HepG2細(xì)胞的增殖及對(duì)HepG2中MicroRNA-122、MicroRNA-21表達(dá)的影響.pdf

1、分類號(hào)R575學(xué)校代碼10601密級(jí)公開編號(hào)Y1002060碩士學(xué)位論文苦參素對(duì)L02、HepG2細(xì)胞的增殖及對(duì)HepG2中MicrNA122、MicrNA21表達(dá)的影響學(xué)科專業(yè):內(nèi)科學(xué)研究方向:肝癌基礎(chǔ)與臨床研究生姓名:向發(fā)良學(xué)號(hào):Y1002060學(xué)位類型科學(xué)學(xué)位導(dǎo)師姓名:黃贊松教授二O一三年五月目錄論文部分................................................................

2、...............................................1苦參素對(duì)L02、HepG2細(xì)胞的增殖及對(duì)HepG2中的MicrNA122、MicrNA21的表達(dá)的影響........................................................................1中文摘要............................................

3、.............................................................1英文摘要........................................................................................................3英漢縮略詞對(duì)照表.........................................

4、................................................6前言................................................................................................................71材料與方法..................................................

5、.............................................101.1實(shí)驗(yàn)材料和儀器...................................................................................101.1.1細(xì)胞株......................................................................

6、.......................101.1.2主要試劑及耗材.............................................................................101.1.3主要儀器、設(shè)備..............................................................................111.2細(xì)胞培養(yǎng).....

7、...........................................................................................121.2.1細(xì)胞計(jì)數(shù).........................................................................................121.2.2細(xì)胞復(fù)蘇..................

8、.......................................................................121.2.3細(xì)胞傳代.........................................................................................131.2.4細(xì)胞凍存......................................

9、...................................................131.3MTT法....................................................................................................141.3.1MTT實(shí)驗(yàn)原理..............................................

10、...................................141.3.2MTT實(shí)驗(yàn)步驟.................................................................................141.4流式細(xì)胞術(shù)................................................................................