魚類融合抗菌肽重組表達(dá)載體構(gòu)建及蛋白表達(dá)研究.pdf

1、浙江大學(xué)生命科學(xué)學(xué)院碩士學(xué)位論文魚類融合抗菌肽重組表達(dá)載體構(gòu)建及蛋白表達(dá)研究姓名：施科云申請學(xué)位級別：碩士專業(yè)：細(xì)胞生物學(xué)指導(dǎo)教師：項(xiàng)黎新20090615浙江大學(xué)碩士學(xué)位論文AbstractEndogenousantimicrobialpeptidesareoneofthemostimportanteffectorsintheinnateimmunityAntimicrobialpeptideshaveabroadspectrumant

2、imicrobialactivityandthebacteriaarehardlyresistanttothemSoitprovidesanovelnativeantibioticinpreventionandcureofthefishdiseasesInthefirstpartoftheresearchincombinationoftheCOIItmoncharacterbetweentheeukaryoticandprokaryot

3、icexpressionsystem，wedesighedandsynthesizedthefusiongene，cascadeconnectionofthreemarineantimicrobialpeptide，whichiscoinedasMoronecidin—HepcidinPleurocidinAftersuccessfullyconstructedtheeukaryoticandprokaryoticexpressionv

4、ectorscontainingthefusionantimicrobialpeptide，weexpressedthefusionproteinintheeukaryoticandprokaryoticexpressionsystemstoobtainanewantimicrobialbioproductInthesecondpartoftheresearch，weexpressedtheextracellularpartofTLRl

5、proteininprokaryoticexpressionsystemMeanwhile，utilizingthemodifiedmethodsofoverlappingextensionPCRweconstructedthefusiongeneharboringthehepcidingeneandextracellularpartofTLRlgeneAfterrestructuringtherecombinantplasmidwhi

6、chwasinsertedwimthefusiongene，weexpressedthefusionproteinintheprokaryoticexpressionsystemThisresearchpresentsanovelmethodtogainefficientandtargetingeffectantimicrobialpeptidesKeywords：fish；antimicrobialpeptide；Toll1ikere