牛瑟氏泰勒蟲P33表面蛋白基因的原核表達.pdf

1、延邊大學(xué)碩士學(xué)位論文牛瑟氏泰勒蟲P33表面蛋白基因的原核表達姓名：李春花申請學(xué)位級別：碩士專業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：許應(yīng)天20070420延邊大學(xué)碩士學(xué)位論文一AbslradProkaryoticexpressionofP33majorsurfaceprotein弋r呱Theieriasergent|Abstract7礙PjJ8，j口sj5isoneofbloodprotozoaldisease，causedby珀ef，erjafami

2、ly，砌ejJP，jagenus，勱P(guān)j』e一日sPr船門￡jItmultipliesincattleintererythrocyteswhichresultsinseriouseconomic10ssesinaniⅡ口lhusbandryRecentyears，勱P(guān)jJBrjDsjsDcc山：陽如d啪sf丑占亡即d如c只bothdiseaserate8ndfatalityratearehighinepide皿icarea，whichc

3、ausedaboardimportanceof釉rldZ鉑聊療￡jP33proteinisoneofZ鮚印打fjmajorsurfaceprotein，andhasstrongi砌unogenicityThereishydrophobiemembraneanchorontheCend，andsignalpeptideontheNendofP33genesequenceApairofsynthetic01igonucleotideprim

4、erswhichdesignedaccordingtothegeneencodingP33majorsurfaceproteinof勱1日一J610ajP，箸’鯽亡‘wereusedto鋤plifytheparasiteDNAfromthebloodofTsergenti—infectedcattleHom0109yofnucleotidesequencebetweentheclonedP33geneandthepublishedseq

5、uencefromGenBankwas99％，whichprovedthepurposegenefragmentwasP33majorsurfaceproteinof7曲Pj』e，jasP，g冒門jP33majorsurfaceproteingenewas1igatedintopET一28a()vectorandwascertifiedbyPCRaJTIplificationanddigestionwithendonuclease，wh

6、ichprovedaprokaryoticexpressionvectorthatcontainingthepET一28拿一P33wasconstructedsuccessful1yTheexpressedP33proteinm01ecure303ku，anditwas43％ofthetotalbacteriumproteinItexistsincytorrhyctesstatusTheconstructedpET一28a—P33vec