熱休克預處理對視網(wǎng)膜色素上皮細胞氧化損傷的影響.pdf

1、[ Objective] To study the effect of heat shock pretreatment on the express of HSP27 in retinal pigment epithelium cells and the effect on H2O2-induced apoptosis of retinal pigment epithelium (RPE)cells. [Methods]

2、 The cultivate retinal pigment epithelium cells were divided into 4 groups: control group,H2O2 injury group, heat shock pretreatment group and H2O2 injury after heat shock pretreatment group; cells Group 2 and 4 are c

3、ulture in 42℃ water for 20r,in,then set in the incubator, after diffent time(0h, 2h,4h,8h,12h,24h) to break off culture and used in experiment;cells Group 3 and 4 are exposed to 600μmol· L-1 H2O2 to induce retina pigmen

4、t epithelium cells oxidation injury; The percentage of apoptotic cells were analyzed by Flow Cytometry; heat shock proteins were detected by immunochemistry. [ Results] There are mild express of hsp27 in retina p

5、igment epithelium cells. After heat shock pretreatment the express of hsp27 are enhancement in different degree. After heat shock pretreatment 2 hours the express begin to heighten, after 4h and 8h the express reach to

6、the peak ,and after 12h and 24h the express were begin to decline. the apoptosis of Group 3 are obviosly ,the apoptosis of Group 4 are descent in different degree. Heat shock pretreatment can inhibit the H2O2-induced apo

7、ptosis of RPE cells obviously. [ Conclusion ] Heat shock pretreatment can induce the overexpression of hsp27 and against the apoptosis induced by H2O2. the main mechanism perhaps was via induce the overexpression