產(chǎn)共軛亞油酸植物乳桿菌的篩選及發(fā)酵工藝研究.pdf

1、南昌大學(xué)碩士學(xué)位論文產(chǎn)共軛亞油酸植物乳桿菌的篩選及發(fā)酵工藝研究姓名：林江申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：曾哲靈熊濤20081201AbstractABSTRACTConjugatedlinoleicacid(CLA)haverecentlyattractedmuchattentionasanoveltypeofbiologicallybeneficialfunctionalfattyacidsSomeisomersofCLAw

2、erereportedtoreducecarcinogenesis，atherosclerosisandbodyfat，enhanceorganismimmunityandpromotegrowthCLAhasbeenwidelyusedinHealthcareandproductionoffoodadditiveThechemicalapproachestosynthesizingCLA，havebeenstudiedearlyand

3、thoroughlyinindustryfields，however，theresultingCLAproductcontainedtoxicsubstances，thecostsofproductpurificationanduseinalargeamountwerethusveryhighInthisresearch，wescreenedLacticacidbacteriumstrainswimhighCLAproducingcap

4、acityfromfoodandoptimizedthefermentationmediumandfermentationconditionsbasedonthefastdeterminationmethodofCLA，andtheresultsofthispaperareasfollowing：1SeveralstrainspossibleproducingCLAwerescreenedfromfermentationChineses

5、auerkrautjuice，lactobinandyogurtThreestrainsproducingCLAwerescreenedfromfermentationChinesesauerkrautjuice，andonestrainswerescreenedfromlactobinandyogurtrespectivelybyusingUVspectrophotometrymethodStrainLJ05，havethehighe

6、stCLAproducingcapacityCLAproducedbystrainU一05contains3215％cis9，transl1C18：2isomerand6885％transl0，cisl2C18：2isomerandtherelativeamountofcis9，trans11C18：2andtranslO，cisl2C18：2isomerinextractare081％and179％detectedbyGasChrom

7、atographymethod2IdentifystrainLJ一05byphysiologyandbiochemistryapproaches，thenidentifystrainLJ一05byBiologMicrobialIdentificationSystem(BMIS)，comparemeritanddemeritofthistwoidentificationmethod，andstrainLJ一05wasidentifieda

8、sLactobacillusplantarumfinally3ThefermentationmediumandfermentationconditionstoproduceCLAofLactobacillusplantarumLJ一05werestudiedbyusingorthogonalexperimentandresponsesurfacemethodTheoptimummediumconsistedofglucose4％，pep