30514.柳樹葉原花青素的提取純化及其功能研究

1、西南大學(xué)碩士學(xué)位論文柳樹葉原花青素的提取純化及其功能研究姓名：羊芹申請學(xué)位級別：碩士專業(yè)：遺傳學(xué)指導(dǎo)教師：唐云明20090501abstractThestudyonextraction，purificationandfunctionlityofproanthocyanidinfromwillowleavesDirection：CellularandmoleculargeneticSupervisor：ProfTangYunmingMas

2、terCandidate：YangQinAbstractProcyanidinsareoneofphenoliccompoundswhichcanfoundinmanyplantsProcyanidinsarealsopolyedbycatechin，andepicatechinindifferentnumbersTheyhavebroadbiologi【calandpharmacologicaleffectsItcanactasana

3、ntioxidantremovesfreeradicalsexcellentlyItalsohasexcellentsecurityWillowareoneoftheornamentalplantswhichdistributedinourcountrywideIv啊艙procyanidinsarehigherinwillowleavesrelativelyInthispaperwestudiedtheoptimumextracting

4、conditionandtheoptimumrefinedmethodofproanthocyandinsfromtheleavesofwillowandtheantioxidantabilityandstabilityofwillowleavesextracts7艙technologyofextractproanthocyandinsfromtheleavesofwillowwasstudied刀犯effectsoffivefacto

5、rssuchasalcoholconcentrationtemperatureofextractiontimeofextraction，ratioofsolidliquidandsolvent，TheamountofacidwereinvestigatedThroughorthogonaltest，theoptimumconditionsofextractionwereobtainedonthebasisofsinglefactorte

6、stasfollows：70％ethanolassolvent，ratioofsolidliquid1：20，80℃for2handwith025％acidTheextractionpercentofproanthocyandinsfromtheleavesofwillowwas11984mg／gontheoptimumconditions(pergramdrypowderofwillowleavesextract11984mgproa

7、nthocyanidnis)Andthen，handlestheseextractswithltimevolumepetroleumaetherand3timesvolumeacetone，thepurityofproanthocyanidinreaches159％，andtheyieldofproanthocyanidinsreaches86％bytheprocessTheseextractswerefurtherpurifiedby

8、AB一8macroporousadsorbentresinTheoptimalconditionsofpurificationwerethat：theabsorptivevelocityis2bedvolumesperhour(2BV／h)，andmadethisloadingfor3timestofullyabsorbedElutingwithwateratthedesorpitvevelociteof2BV／htilltheelua

9、ntbecomenocolorAndthenelutewith40％EtOHatthevelociteof16mL／mingathertheeluant2BVIIlthiswayTheyielfofproanthocyanidinsreaches711％andthepurityofproanthocyanidinsreaches583％111emeandegeeofpolymerization(DP)ofproanthocyanidin