胚胎肢芽提取物對周圍神經(jīng)損傷后脊髓前角運動神經(jīng)元的保護作用

1、胚胎肢芽提取物對周圍神經(jīng)損傷后脊髓前角運動神經(jīng)元的保護作用更新日期：20100908劉紅光洪光祥王發(fā)斌何善述【摘要】目的研究周圍神經(jīng)損傷后脊髓前角運動神經(jīng)元內(nèi)酶學(xué)的變化并探索從大鼠胚胎肢芽中提取的活性物質(zhì)(ELBE)對神經(jīng)元的保護作用。方法切斷大鼠坐骨神經(jīng)在近端套接單盲端硅膠管囊單純損傷組囊內(nèi)注滿等滲鹽水保護組內(nèi)注滿ELBE。分別于術(shù)后1、4天1、2、3、4周定量測量每克脊髓組織內(nèi)乙酰膽堿脂酶(AchE)和酸性磷酸酶(ACP)的含量。結(jié)

2、果AchE于損傷后1天開始下降2周降至最低以后逐漸回升ACP于損傷后1天開始升高1周升至最高以后逐漸下降用ELBE處理的動物上述兩種酶變化的幅度較小整個過程也比較平穩(wěn)。結(jié)論ELBE能通過緩沖神經(jīng)損傷后神經(jīng)元內(nèi)酶學(xué)的變化起到保護神經(jīng)元的作用從而減輕神經(jīng)元損傷的程度減少神經(jīng)元死亡的數(shù)量有利于神經(jīng)再生。【關(guān)鍵詞】酶胚胎肢芽提取物神經(jīng)元神經(jīng)損傷ProtectiveEffectofEmbryonicLimbBudsExtractonMotNeur

3、onsinAnteriHnofSpinalCdAfterSciaticNerveInjuryLIUHongguangHONGGuangxiangWANGFabinetal.Dept.ofHsurgeryUnionHospitalTongjiMedicalUniversityWuhan430022【Abstract】AimTostudytheenzymicchangeprotectionwithembryoniclimbbudsextra

4、ct(ELBE)ofthemotneuronsinanterihnofspinalcdafterthesciaticnerveinjury.MethodsSixtyeightSDratswerefreelydividedinto2groupsthentransectedthesciaticnervewithasingleblindsiliconetubewhichwasfilledwithnmalsaline(NS)inthesingl

5、einjurygroupwithELBEintheprotectiongroupwhichcouldbelocallyappliedtotheproximalnervestumplasting4weeks.Activitiesofacetylcholinesterase(AchE)acidphosphatase(ACP)inventralspinalcdofSDratswerequantitativelymeasuredin1stday

6、4thday1stweek2ndweek3rdweek4thweekafterthesciaticnerveinjury.ResultsTheratstreatedwithNSshowedAchEdecreased1dayafterthenervetransectionreacheditslowestpointin2weeksthenrecoveredgraduallyACPincreased1dayafterthenerveinjur

7、yreachedpeakin1weekthenrecoveredgraduallyTheratsprotectedwithELBEshowedrelativelysmoothchangeofthetwokindsofenzymes.ConclusionELBEcanprotectthemotneuronsinanterihnofspinalcdthroughresistingthechangeofAchEACP.【Keywds】Enzy

8、meEmbryoniclimbbudsextractNeuronsNerveinjury周圍神經(jīng)損傷后不僅遠(yuǎn)端神經(jīng)發(fā)生變性反應(yīng)而且中樞相關(guān)神經(jīng)元也出現(xiàn)變性反應(yīng)使一定數(shù)量的神經(jīng)元發(fā)生損傷性死亡［1］。如坐骨神經(jīng)橫斷常致脊髓前角運動神經(jīng)元發(fā)生軸突反應(yīng)形態(tài)上表現(xiàn)為尼氏體溶解核偏位等改變［2］。筆者從測定顯示脊髓前角運動神經(jīng)元活性的標(biāo)志酶乙酰膽堿脂酶(AchE)和發(fā)生損傷性反應(yīng)的標(biāo)志酶酸性磷酸酶(ACP)出發(fā)研究周圍神經(jīng)損傷后相關(guān)神經(jīng)元內(nèi)酶學(xué)的

9、變化并探索從大鼠胚胎肢芽中提取的活性物質(zhì)(embryoniclimbbudsextractELBE)對神經(jīng)元的保護作用。材料與方法(0.200.04)Ug脊髓組織。坐骨神經(jīng)損傷后1天開始上升1周升至最高以后逐漸下降加ELBE處理后ACP上升緩慢幅度變小4天后即開始下降整個過程波動不大。兩者差別有非常顯著性意義(表2)。表2ACP含量的變化(Ug脊髓組織s)組別術(shù)后時間1天4天1周2周4周6周ELBE保護組0.230.020.290.03