大環(huán)內脂類耐藥性對空腸彎曲桿菌毒力和適應性的影響.pdf

1、分類號華中農業(yè)大學博士學位論文大環(huán)內醋類耐藥性對空腸彎曲桿菌毒力和適應性的影響ImPactofMaerolideResistanceontheViruleneePr0PertiesandFitnessinCan耳)J，tobacterjejuni研究生:vassirAliA一moftiAhmed指導教師:袁宗輝教授指導小組:戴夢紅副教授郝海紅講師專業(yè):基礎獸醫(yī)學獲得學位名稱:農學博士研究方向:生物化學與分子生物學獲得學位時間:2011年

2、6月華中農業(yè)大學動物醫(yī)學院二O一一年六月2.2.8Illustrativecaleulationsofseleetionrateeonstant(R)，MalthusianParameter(M)，numberofthedoubling(D)relativefitness(W)ofeachcompetit...................................................................

3、.................................……232.2.9Heatshocktoleranceassay.................................................................……242.2.10C01onizationofbalbcmice.........................................................

4、.....……252.2.11Statisticalanalysis............................................................................……262.3Resuhs.................................................................................................

5、..........……262.3.1Deter們ninationofm以ationsinmaero【ideresistantm以ants...............……262.3.2Determinationofminimali雨bityconeentration(MIC)................……282.3.3Growthrateofsl221straineomParedtoSTY，SEandSTIstrains..……

6、292.3.4Relativefitnessofsl22lstraineomParedtoSTY，SEandSTIstrains.…302.3.5Heatshocktoleranceassay.................................................................……322.3.6Colonizatlonofbalbcmicem0del......................

7、...............................……332.4Oiscussion......................................................................................................……353.ImPaetoferythromyeinresistaneeontheViruleneeProPertiesandfitness

8、ofCa刀聊夕lobacterjej’uni..................................................................................................……383.1Introduction......……‘........................................................................

9、.............……383.2Materialsandmethods.......................................................……，.....................……403.2.1Bacteria1strainandcu1turec0nditi0ns...............................................……403.2.2In

10、vitroinduction................................................................................……403.2.3AntimicrobialsuscePtibilitytesting....................................................……403.2.4Cjej’unjgenomieDNAextr

11、actiondeterminationofmutationsinmacrolideactionsites.....................................................……，......................……403.2.5Resistaneetobilesalts(sodiumdeoxycholate).……，..........................……413.2.6I