肝細胞生長因子對高糖環(huán)境下人視網(wǎng)膜色素上皮細胞的影響.pdf

1、山西醫(yī)科大學碩士學位論文肝細胞生長因子對高糖環(huán)境下人視網(wǎng)膜色素上皮細胞的影響姓名：李曉燕申請學位級別：碩士專業(yè)：內科學（內分泌及代謝?。┲笇Ы處煟豪钆d20090315山西醫(yī)科大學覃士學位論文TheeffectofHepatocytegrowthfactoronhumanretinalpigmentepithelialcellsunderhighglucoseAbstractObjective：Toinvestigatetheeffect

2、sofHepatocytegrowthfactor(HGF)onproliferationandtheexpressionofvascuarendothelialgrowthfactor(VEGF)andpigmentepitheliumderivedfactor(PEDl0ofhumanretinalpigmentepithelial(hRPE)cellsculturedwithhighglucoseinvitroMethods：Th

3、ehRPEcellsweredividedintonormalglucosegroup(55rnmol／Lglucose，)，highglucosegroup03mmol／Lglucose)，highglucoseplusHGFgroup03mmol／Lglucose50ng／mlHGF)12ll’24ll，48Il，72Il’96hlaterthecellproliferationwasobservedbyMTTassayTheILR

4、PEweredividedintonormalglucosegroup(55nunol／Lglucose，NG)，highglucosegroup03mmol／Lglucose，HG)，highglucoseplusadiponectingroup(33mmol／Lglucoseplus50ng／mlHGF)12h24h，48h72h，96hlater，thelevelofVEGFandPEDFwasmeasuredbyenzymeli

5、nkedimmunosorbcntassay(ELISA)ResuRs：ThetreatmentofhRPEcellswith33mmol／Lglucosecausedaobviousdecreaseofcelluarroliferationafter24h48h，72hand96hComparedwithhighglucosegroup，HGFgroupafter12h2413detectionofcellviabilityhadno

6、significantchangesfF005)；48h72h96hlater，cellviabilityhadsignificantchangesifO05)；Comparedwithnormalglucosegroup，theVEGFlevelsignificantlyincreasedandPEDFsignificantlydecreasedinhighglucosegroupafter24h48h72hand96hCompare

7、dwithhilghglucosegroup，theVEGFlevelsignificantlyincreasedandPEDFsignificantlydecreasedinhighglucoseplusHGFgroup(50ng／m1)(P005)Conclusion：ThehighglucoseinhibitstheproliferationactivityofhRPEandincreaseVEGFanddecreasePEDF5

8、0ng／mlHGFCanpromotetheproliferationactivityofhRPEandincreaseVEGFanddecreasePEDFHGFCanregulatethebalanceofVEGFandPEDFtopromotetheformationofnewbloodvesselsindiabeticrctinopathy(DR)Keywords：Highglucose，Retinalpigmentepithe