UVB對體外培養(yǎng)皮膚干細胞某些標記分子表達的影響研究.pdf

1、福建醫(yī)科大學碩士學位論文UVB對體外培養(yǎng)皮膚干細胞某些標記分子表達的影響研究姓名：王菁申請學位級別：碩士專業(yè)：皮膚病與性病學指導教師：程波20100401英文摘要Purpose：toinvestigatethechangesofmoleculalmarkersinculturedskinstemcellsexposuretoUVBinvitroMethods：Theskinstemcellswereisolatedandcultured

2、accordingtotheiradheredabilityandidentifiedbyK15andIMntegrinexpressionTheskinstemcellswereexposuredintheUVBirradiationTheUVB’Speakvaluewas305nmandthedosagesWas10mJ／cm2ThemorphologicalchangesofskinstemcellsafterUVBirradim

3、ion(experimentalgroup)ornot(controlgroup)wereobservedThechangesofCD34、betacatenin、P53ofskinstemcellsweredetectedbyimmunohistochemistyandcomparedinUVBexposuregroupornotResults：①Theskinstemcellsweresuccessfullyseparatedand

4、culturedTheyexpressedcharacteristicskinstemcellmarkerK15andpintegrin@CellsofcontrolgrouphadadensedensityTheywereroundorpolygonthecellswerefoundtohavehighN／CratiosandthedividingcellscanbefoundThemorphologywasclearandthecy

5、toplasmWaswelldistributedIncontrast，theskinstemcellsinexperimentalgroup，grewslowlythecellmorphologywasdeformitiesandanomalous，vacuolesweredetectedincytoplasmThecellswerefoundtohavelowN／Cratiosaproportionofcellswerekaryop

6、yknosisorapoptosis@Inthecontrolgroupofskinstemcells，betaCateninwereexpressedpremodinantlyinthecellmembraneorcytoplasmanditsmembraneandnucleusstainedpositiverateswere6474％，84％；P53wereexpressedmainlyinthecytoplasmandtheirn

7、ucleistainedpositiveratewere69％Incontrast，intheexperimentalgroupofskinstemcells，betaCateninexpressionmainlyinthecytoplasmandthenucleus，thecellmembraneandstainedpositiverateswere6474％，0P53expressionmainlyinthenucleus，then