馬立克氏病病毒UL13蛋白激酶的體外表達(dá)及抗體制備.pdf

1、揚(yáng)州大學(xué)碩士學(xué)位論文馬立克氏病病毒UL13蛋白激酶的體外表達(dá)及抗體制備姓名：蘇鈺文申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：劉岳龍秦愛建20090501揚(yáng)州大學(xué)碩士學(xué)位論文InVitroexpressionandantibodypreparationofMarek’sII—diseaseVims(MDV)UL13proteinkinaseMSCarldidate：ⅥJwenSuSupeⅣisor：Y，uelongLiuAijianQi

2、nAbstractBaCtoBac@BaculovimsExpressingSystemis蜀汜quemly印pliedt0generateheterogonousproteiIlSo、vingt0itsspecialcharactersofhi曲leVelsofheterogonousgeneexpressionandhighbioaCtiVi吼aIldmepllIifiedproteinarecontributedtot11e觚al

3、ysisofproteinbio如nctionThebiofhnctionofconservedhe印esViralproteinl(inaSes(CHPKs)inherpesVimsareoneoftllehottopicofhe印esVims’researchT11erolesofdi毹remCHPKsplaydifrerently鋤ongdifrerenthe印esViridaeInthis殉Jdy，BaCtoBac@Baculo

4、viruSExpressingSystemw舔印pIiedtoexpressthe朋口旭七≥如P儺P1，驢姍(MDV)UL13proteinl(inasewhichisamemberofCHPKsThreerecombinantbaculovimsesincludingrBacHis—ULl3，rBac—HisEGFPandrBacHisWereconstructedSuccess如llyOpt婦alexpressionconditio

5、nsandharVesttimeforMDVULl3prmeinl(inauseauldEGFP、Vereconfi肌edbyoptimizationassaysInthisstud弘也e叫ficationplatfomofBactoBac@BaculovimsExpressingSystemwaSpreliminarilyestablishedbasedonBaCtoBac@HTvectorsBesides，theessentialm

6、atedalsforMDVULl3proteinkinaSebio—functionresearchwereobtainedToobserVet11een’ectSontheViral黟owthofMDVaLssays、ⅣereperfoHIledthrou曲MDVUL13proteinkinaSeuptal【eTheresultsshowedthatMDVUL13proteinkinaSehadnosi鰣ficante髓ctsontl

7、leplaquesizeofaVeD，Vimlent(w)MDvlstrain—RB一1BPolyclonalamibodyagainsttlle血UUL13ORFwaSpreparedbygeneilIl】mImizationAntibodiesagainstMDVULl3proteinkinaSegeneratedviadifrerentmethods、Ⅳere印pliedtodetectedMDVULl3proteinkinaSe